Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples.
Comparison of polymerase chain reaction and culture methods for detection of Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in subgingival plaque samples.
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聚合酶链反应和培养方法检测龈下菌斑样品中伴放线放线杆菌和牙龈卟啉单胞菌的比较。
DOI：
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发表时间：
1996
影响因子：
3.5
通讯作者：
D. Kinane
中科院分区：
文献类型：
--
作者：
M. Riggio;T. Macfarlane;D. Mackenzie;A. Lennon;Andrew Smith;D. Kinane
本研究采用聚合酶链式反应(PCR)和常规培养方法对牙周病患者的菌斑标本中的主要牙周病原菌进行了检测。对43例患者的170个菌斑标本进行了分析，其中40例(24%)用聚合酶链式反应检测到伴生放线菌，18例(11%)用常规培养法检出伴生放线菌和牙龈假单胞菌。至少1个牙周标本中伴生放线菌的比例，聚合酶链式反应检测为17/43(40%)，培养法为13/43(30%)；牙龈假单胞菌为12/43(28%)，培养法为9/43(21%)。只有5个样本，来自3个病人，同时含有放线菌伴生菌和牙龈假单胞菌。结论：与常规培养方法相比，聚合酶链式反应技术能更准确地鉴定牙周菌斑标本中的牙周病原菌，并具有较高的检出频率。
In this study, the major periodontal pathogens Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis were detected in subgingival plaque samples from patients with periodontal disease by polymerase chain reaction (PCR) and conventional culture methods. 170 plaque samples from 43 patients were analysed; A. actinomycetemcomitans and P. gingivalis were each detected in 40 (24%) of samples by PCR, whereas conventional culture methods detected A. actinomycetemcomitans and P. gingivalis in 25 (15%) and 18 (11%) of samples, respectively. The proportion of patients carrying A. actinomycetemcomitans in at least 1 sampled periodontal site was 17/43 (40%) by PCR and 13/43 (30%) by culture; for P. gingivalis this was 12/43 (28%) by PCR and 9/43 (21%) by culture. Only 5 samples, from 3 patients, harboured both A. actinomycetemcomitans and P. gingivalis. It is concluded that PCR is more accurate than conventional culture methods for identification of these periodontal pathogens in subgingival plaque samples and has a higher frequency of detection.
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影响因子：
56.9
作者：
HOLT, SC;EBERSOLE, J;KORNMAN, KS
通讯作者：
KORNMAN, KS
用于识别龈下微生物的非同位素 DNA 探针。
影响因子：
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作者：
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通讯作者：
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手术加强力霉素对幼年牙周炎的微生物学和临床效果。
影响因子：
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作者：
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通讯作者：
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DNA探针检测龈下菌斑中的腐蚀艾肯氏菌、直肠沃利氏菌和具核梭杆菌。
影响因子：
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作者：
Lippke,JA;Peros,WJ;Keville,MW;Savitt,ED;French,CK
通讯作者：
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通过聚类分析和脱氧核糖核酸杂交对伴放线放线杆菌和嗜血嗜血杆菌进行分类和鉴定。
影响因子：
3.5
作者：
Tanner,AC;Visconti,RA;Socransky,SS;Holt,SC
通讯作者：
Holt,SC