In vivo volumetric imaging of microcirculation within human skin under psoriatic conditions using optical microangiography.
In vivo volumetric imaging of microcirculation within human skin under psoriatic conditions using optical microangiography.
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DOI：
10.1002/lsm.20977
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发表时间：
2011-02
影响因子：
2.4
通讯作者：
Wang, Ruikang K.
中科院分区：
文献类型：
--
作者：
Qin, Jia;Jiang, Jingying;An, Lin;Gareau, Daniel;Wang, Ruikang K.
关键词：
越来越多的证据表明，血管异常可能在多种皮肤病中起着至关重要的作用，如牛皮癣、葡萄酒斑和皮肤癌。为了提高我们对这些皮肤状况中血管参与的理解，需要一种能够评估体内皮肤组织床内3D微循环的非侵入性成像方式。本研究旨在证明超高灵敏度光学微血管造影（UHS-OMAG）在正常和银屑病条件下是否可以实现皮肤微循环的三维可视化和微血管密度的量化。采用1310nm波长的UHS-OMAG系统对人体皮肤微循环进行体内成像。该系统的空间分辨率为~10µm ×20µm（轴向×横向），以每秒280帧的速度运行，获取3D成像数据集，以表示扫描皮肤组织体积内的形态和毛细血管水平微血管血液灌注。该系统对血流的灵敏度低至~4µm/s。利用该系统，我们对一名患有稳定斑块型银屑病的志愿者进行了皮肤成像实验。对正常皮肤和病变皮肤的微循环和结构信息进行定性和定量比较。UHS-OMAG能够区分正常皮肤和银屑病皮肤的微循环。三维光学图像显示银屑病病变皮肤血管伸长和致密网络，其外观在正常皮肤中没有观察到。根据一个受试者的结果，统计分析表明，银屑病病变皮肤内的血管密度高于正常皮肤。UHS-OMAG是一种具有高速、高灵敏度、无创的皮肤微循环成像的宝贵工具，因此可能在未来的临床诊断和治疗人类皮肤病（如牛皮癣）中发挥有用的作用。
There is a growing body of evidence suggesting that vascular abnormalities may play crucial role in several dermatologic diseases, such as psoriasis, port wine stain and skin cancer. To improve our understanding of vascular involvement in these skin conditions, there is a need for a non-invasive imaging modality capable of assessing 3D microcirculations within skin tissue beds in vivo. This study aims to demonstrate whether ultrahigh sensitive optical microangiography (UHS-OMAG) is feasible to visualize skin microcirculations in 3D and to quantify microvascular vessel density under normal and psoriatic conditions in vivo. An UHS-OMAG system operating at 1310nm wavelength was used for in vivo imaging of microcirculation in human skin. The system has a spatial resolution of ~10 µm ×20 µm (axial × lateral), running at 280 frames per second to acquire 3D imaging dataset to represent morphology and capillary level microvascular blood perfusion within the scanned skin tissue volume. The sensitivity of the system to the blood flow is as low as ~4 µm/s. With this system, we performed the imaging experiments on the skin of a volunteer with stable plaque-type psoriasis. The microcirculation and structural information of normal and diseased skins were compared both qualitatively and quantitatively. The UHS-OMAG is capable of differentiating the microcirculation within the normal skins from that in the psoriatic skins. The 3D optical images show the blood vessel elongation and the dense network in the psoriatic lesion skin, the appearance of which is not observed within the normal skin. Based on the results obtained from one subject, the statistical analyses show that higher blood vessel density presented within the psoriasis lesion skin than that of the normal skin. UHS-OMAG can be a valuable tool for imaging skin microcirculations non-invasively with high speed and high sensitivity, and therefore may have a useful role in future clinical diagnosis and treatment of dermatologic diseases such as psoriasis in human subjects.
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影响因子：
3.8
作者：
An L;Qin J;Wang RK
通讯作者：
Wang RK
影响因子：
168.9
作者：
BARKER, JNWN
通讯作者：
BARKER, JNWN
影响因子：
2.4
作者：
Murray, AK;Gorodkin, RE;King, TA
通讯作者：
King, TA
影响因子：
2.3
作者：
Humbert, Philippe;Sainthillier, Jean-Marie;Aubin, Francois
通讯作者：
Aubin, Francois
影响因子：
3.8
作者：
Srinivasan VJ;Sakadzić S;Gorczynska I;Ruvinskaya S;Wu W;Fujimoto JG;Boas DA
通讯作者：
Boas DA