Alkylacetylglycerophosphocholine effects on the metabolism of phospholipids in rabbit platelets: effects of extracellular Ca2+ and prostacyclin.
Alkylacetylglycerophosphocholine effects on the metabolism of phospholipids in rabbit platelets: effects of extracellular Ca2+ and prostacyclin.
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烷基乙酰甘油磷酸胆碱对兔血小板中磷脂代谢的影响：细胞外 Ca2 和前列环素的影响。
DOI：
10.1016/0003-9861(86)90599-0
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发表时间：
1986
影响因子：
3.9
通讯作者：
Hanahan,DJ
中科院分区：
文献类型：
--
作者：
Tokumura,A;Kramp,W;Hanahan,DJ
烷基乙基甘油磷酸胆碱（AGEPC）刺激32p标记溶血磷脂酸在洗涤兔血小板中的形成依赖于细胞外Ca2+。与同一激动剂诱导的肌醇磷脂的降解和磷脂酸的产生相比，其积累速度较慢，需要较高浓度的AGEPC。这些结果表明，溶血磷脂酸的形成与AGEPC对兔血小板的初次活化没有直接关系。AGEPC诱导肌醇磷脂的优先降解顺序如下：磷脂酰肌醇4,5-二磷酸>磷脂酰肌醇4-磷酸>磷脂酰肌醇。用前列环素或乙二醇双（β-氨基乙醚）N,N ' -四乙酸（EGTA）预处理血小板影响肌醇磷脂的降解和随后磷脂酸的生成。在用前列环素和EGTA预处理的血小板中观察到这些代谢变化的协同抑制作用。将这些结果与前列环素和EGTA对AGEPC诱导的血清素释放的影响进行比较，并就AGEPC诱导的磷脂代谢变化在血小板活化机制中的可能作用进行探讨。
Alkylacetylglycerophosphocholine (AGEPC) stimulation of32P-labeled lysophosphatidic acid formation in washed rabbit platelets was dependent on extracellular Ca2+. Its accumulation was slower and required a higher concentration of AGEPC in comparison to the degradation of inositol phospholipids and production of phosphatidic acid induced by the same agonist. These results suggest that the formation of lysophosphatidic acid is not directly related to the primary activation of rabbit platelets by AGEPC.AGEPC elicited a preferential degradation of inositol phospholipids in the following order: phosphatidylinositol 4,5-bisphosphate > phosphatidylinositol 4-phosphate > phosphatidylinositol. The degradation of inositol phospholipids and subsequent production of phosphatidic acid were affected by pretreatment of platelets with prostacyclin or ethylene glycol bis(β-aminoethyl ether)N,N′-tetraacetic acid (EGTA). Synergistic inhibitions of these metabolic changes were observed in the platelets pretreated with both prostacyclin and EGTA. These results were compared with effects of prostacyclin and EGTA on serotonin release induced by AGEPC, and the possible roles of metabolic changes in phospholipids induced by AGEPC are discussed with respect to the mechanism of platelet activation.
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人血小板与磷脂血小板激活因子的特异性结合。
影响因子：
4.4
作者：
F. Valone;E. Coles;V. R. Reinhold;E. Goetzl
通讯作者：
E. Goetzl
乙酰甘油醚磷酸胆碱（AGEPC；血小板激活因子）诱导的兔血小板刺激：磷脂酸水平、45Ca2 摄取和 [3H]5-羟色胺分泌之间的相关性。
影响因子：
3.9
作者：
Shukla,SD;Hanahan,DJ
通讯作者：
Hanahan,DJ
血小板激活因子 (PAF) 诱导五种血小板聚集、释放和脱敏
影响因子：
6.7
作者：
D. Cargill;D. Cohen;R. Valen;J. Klimek;R. Levin
通讯作者：
R. Levin
磷脂诱导的人血小板活化：钙通道阻滞剂和钙螯合剂的作用。
影响因子：
7.5
作者：
D. Macintyre;A. Shaw
通讯作者：
A. Shaw
1-O-烷基-2-乙酰基-sn-甘油基-3-磷酸胆碱（AcGEPC）和棕榈酰-溶血磷脂酸酯在人血小板对胶原蛋白和凝血酶的反应中的作用
影响因子：
3.5
作者：
T. Hallam;M. Scrutton;R. Wallis
通讯作者：
R. Wallis