Small intestinal submucosa gel as a potential scaffolding material for cardiac tissue engineering.
Small intestinal submucosa gel as a potential scaffolding material for cardiac tissue engineering.
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DOI：
10.1016/j.actbio.2009.10.048
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发表时间：
2010-06
影响因子：
9.7
通讯作者：
Wang, Yadong
中科院分区：
文献类型：
--
作者：
Crapo, Peter M.;Wang, Yadong
关键词：
心脏组织工程通常利用富含蛋白质的支架材料和生长因子来改善体外和体内的心脏组织功能。本初步研究的目的是：(1)研究猪小肠粘膜下层凝胶（SIS凝胶）在心脏组织工程中的潜力；(2)比较SIS凝胶和Matrigel（一种肿瘤来源的基准材料）对组织的功能。将新生大鼠心肌细胞与SIS凝胶或Matrigel结合，在聚癸二酸甘油组成的多孔弹性支架上培养13天。通过每日两次测量收缩率来评估组织功能。H&E染色定性比较组织形态。通过图像分析比较归一化肌钙蛋白T表达（肌钙蛋白T:DNA）。在第8-11天，SIS凝胶构建物的收缩率显著高于Matrigel构建物。与Matrigel构建体相比，SIS凝胶构建体的正常化肌钙蛋白T表达明显更高。综上所述，本研究表明：(1)SIS凝胶可用于制造可收缩的工程心脏组织；（II）根据本研究进行的基本体外检查，SIS凝胶产生的工程心脏组织具有更高的生理收缩率和更高的表型蛋白表达。
Cardiac tissue engineering typically utilizes protein-rich scaffolding materials and growth factors to improve cardiac tissue function in vitro and in vivo. The objectives of this preliminary study were (I) to investigate the potential of porcine small intestinal submucosa gel (SIS gel) in cardiac tissue engineering and (II) to compare the function of tissue based on either SIS gel or Matrigel, a tumor-derived benchmark material. Neonatal rat cardiac cells were combined with either SIS gel or Matrigel and cultured on porous elastomeric scaffolds composed of poly(glycerol sebacate) for 13 days. Tissue function was assessed by measuring contraction rates twice daily. Tissue morphology was compared qualitatively by H&E staining. Normalized troponin T expression (troponin T:DNA) was compared using image analysis. SIS gel constructs contracted at significantly higher rates than Matrigel constructs on days 8–11. Normalized troponin T expression was significantly higher in SIS gel constructs compared to Matrigel constructs. In summary, this research demonstrates that: (I) SIS gel can be used to create contractile engineered cardiac tissue; and (II) SIS gel produced engineered cardiac tissues with a more physiologic contraction rate and higher phenotypic protein expression based on basic in vitro examinations performed in this study.
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影响因子：
4.9
作者：
Gao, Jin;Ensley, Ann E.;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
5.9
作者：
Heidenreich, PA;McClellan, M
通讯作者：
McClellan, M
影响因子：
4.9
作者：
Crapo, Peter M.;Gao, Jin;Wang, Yadong
通讯作者：
Wang, Yadong
影响因子：
14
作者：
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通讯作者：
Boccaccini, Aldo R.
影响因子：
37.8
作者：
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通讯作者：
Menasché, P