Core Microbiome and Microbial Community Structure in Coralloid Roots of Cycas in Ex Situ Collection of Kunming Botanical Garden in China.
Core Microbiome and Microbial Community Structure in Coralloid Roots of Cycas in Ex Situ Collection of Kunming Botanical Garden in China.
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DOI：
10.3390/microorganisms11092144
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发表时间：
2023-08-24
期刊：
影响因子：
4.5
通讯作者：
Gong X
中科院分区：
文献类型：
--
作者：
Wang Z;Liu J;Xu H;Liu J;Zhao Z;Gong X
内生菌对于植物的演替和进化至关重要，并且对于抗逆性也至关重要。珊瑚状根是苏铁科植物中发现的一种独特的根结构，起到了抵御恶劣环境的作用，但不同苏铁属物种的核心类群和微生物群落尚未得到深入研究。利用扩增子测序，我们成功阐明了 10 种苏铁属植物的珊瑚根中存在的微生物组，这些苏铁属植物代表了中国苏铁属的所有四个部分。我们发现珊瑚根中的内生细菌，即蓝细菌，主要由Desmonostoc_PCC-7422、Nostoc_PCC-73102和unclassified_f__Nostocaceae组成。此外，Exophiala、Paraboeremia、Leptobacillium、Fusarium、Alternaria 和 Diaporthe 的子囊菌门真菌被确定为核心真菌类群。子囊菌门真菌Nectriaceae、Herpotrichiellaceae、Cordycipitaceae、Helotiaceae、Diaporthaceae、Didymellaceae、Clavicipitaceae和Pleosporaceae被确定为四个部分珊瑚根中的核心科类群。珊瑚根中细菌群落丰度较高，但多样性较低，但物种间差异不显着。与细菌相比，真菌群落表现出更高的复杂性，并且不同物种或部分之间存在多样性。这些核心类群是苏铁物种所有或大多数个体中经常出现的微生物组的一个子集，代表了苏铁保护发展的目标。
Endophytes are essential in plant succession and evolution, and essential for stress resistance. Coralloid root is a unique root structure found in cycads that has played a role in resisting adverse environments, yet the core taxa and microbial community of different Cycas species have not been thoroughly investigated. Using amplicon sequencing, we successfully elucidated the microbiomes present in coralloid roots of 10 Cycas species, representing all four sections of Cycas in China. We found that the endophytic bacteria in coralloid roots, i.e., Cyanobacteria, were mainly composed of Desmonostoc_PCC-7422, Nostoc_PCC-73102 and unclassified_f__Nostocaceae. Additionally, the Ascomycota fungi of Exophiala, Paraboeremia, Leptobacillium, Fusarium, Alternaria, and Diaporthe were identified as the core fungi taxa. The Ascomycota fungi of Nectriaceae, Herpotrichiellaceae, Cordycipitaceae, Helotiaceae, Diaporthaceae, Didymellaceae, Clavicipitaceae and Pleosporaceae were identified as the core family taxa in coralloid roots of four sections. High abundance but low diversity of bacterial community was detected in the coralloid roots, but no significant difference among species. The fungal community exhibited much higher complexity compared to bacteria, and diversity was noted among different species or sections. These core taxa, which were a subset of the microbiome that frequently occurred in all, or most, individuals of Cycas species, represent targets for the development of Cycas conservation.
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影响因子：
2.7
作者：
Wang J;Chen S;Sun R;Liu B;Waghmode T;Hu C
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