Microbial Interkingdom Interactions in Roots Promote Arabidopsis Survival.
Microbial Interkingdom Interactions in Roots Promote Arabidopsis Survival.
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DOI：
10.1016/j.cell.2018.10.020
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发表时间：
2018-11-01
期刊：
影响因子：
64.5
通讯作者：
Hacquard S
中科院分区：
文献类型：
--
作者：
Durán P;Thiergart T;Garrido-Oter R;Agler M;Kemen E;Schulze-Lefert P;Hacquard S
健康植物的根部居住着土壤来源的细菌，真菌和卵菌，它们在不同的生命王国中独立进化。这些微生物如何相互作用，以及这些相互作用在多大程度上影响植物健康，人们知之甚少。我们研究了根相关的微生物群落从三个拟南芥种群，并检测到细菌和丝状微生物真核生物之间的大多数负相关。我们使用无菌拟南芥建立了用于重构实验的微生物培养物收集。在接种单或多王国合成微生物财团的植物中，我们观察到细菌根微生物群对真菌和卵菌群落结构和多样性的深刻影响。我们证明了细菌微生物群对于植物生存和保护免受根源丝状真核生物的侵害是必不可少的。对2,862个二元细菌-真菌相互作用的非原位解卷积，结合植物中的群落扰动实验，表明细菌根际微生物的生物控制活性是一个冗余的性状，它维持了微生物界间的平衡，有利于植物健康。健康植物的根被多王国微生物聚生体细菌根Commensals（BRC）形状真菌和卵菌群落结构BRC保护植物免受真菌和卵菌的侵害BRC的生物控制活性是一种多余的性状，对植物生存至关重要对拟南芥根相关微生物的跨王国分析解释了它们对植物生存的功能贡献。
Roots of healthy plants are inhabited by soil-derived bacteria, fungi, and oomycetes that have evolved independently in distinct kingdoms of life. How these microorganisms interact and to what extent those interactions affect plant health are poorly understood. We examined root-associated microbial communities from three Arabidopsis thaliana populations and detected mostly negative correlations between bacteria and filamentous microbial eukaryotes. We established microbial culture collections for reconstitution experiments using germ-free A. thaliana. In plants inoculated with mono- or multi-kingdom synthetic microbial consortia, we observed a profound impact of the bacterial root microbiota on fungal and oomycetal community structure and diversity. We demonstrate that the bacterial microbiota is essential for plant survival and protection against root-derived filamentous eukaryotes. Deconvolution of 2,862 binary bacterial-fungal interactions ex situ, combined with community perturbation experiments in planta, indicate that biocontrol activity of bacterial root commensals is a redundant trait that maintains microbial interkingdom balance for plant health. Roots of healthy plants are colonized by multi-kingdom microbial consortia Bacterial Root Commensals (BRCs) shape fungal and oomycetal community structure BRCs protect plants against fungi and oomycetes Biocontrol activity of BRCs is a redundant trait and essential for plant survival An interkingdom analysis of the microbes associated with Arabidopsis roots explains their functional contributions to plant survival.
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DOI：
10.1073/pnas.1710455114
发表时间：
2017-10-31
影响因子：
11.1
作者：
Almario J;Jeena G;Wunder J;Langen G;Zuccaro A;Coupland G;Bucher M
通讯作者：
Bucher M
影响因子：
9.5
作者：
Katoh K;Rozewicki J;Yamada KD
通讯作者：
Yamada KD
影响因子：
16.6
作者：
Hacquard S;Kracher B;Hiruma K;Münch PC;Garrido-Oter R;Thon MR;Weimann A;Damm U;Dallery JF;Hainaut M;Henrissat B;Lespinet O;Sacristán S;Ver Loren van Themaat E;Kemen E;McHardy AC;Schulze-Lefert P;O'Connell RJ
通讯作者：
O'Connell RJ
影响因子：
64.8
作者：
Bai, Yang;Mueller, Daniel B.;Schulze-Lefert, Paul
通讯作者：
Schulze-Lefert, Paul
影响因子：
6.6
作者：
Bengtsson-Palme, Johan;Ryberg, Martin;Nilsson, R. Henrik
通讯作者：
Nilsson, R. Henrik