Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells.
Aronia melanocarpa Fruit Bioactive Fraction Attenuates LPS-Induced Inflammatory Response in Human Bronchial Epithelial Cells.
复制标题
DOI：
10.3390/antiox9090816
复制
发表时间：
2020-09-02
期刊：
影响因子：
--
通讯作者：
Yim SV
中科院分区：
文献类型：
--
作者：
Jang BK;Lee JW;Choi H;Yim SV
为了证明黑果腺肋花楸果实提取物的抗炎活性，将人支气管上皮细胞（BEAS-2B）用脂多糖（LPS）处理，并研究了来自黑果腺肋花楸果实的富含花青素的提取物、腺肋花楸生物活性级分（ABF®）的作用。melanocarpa，进行了评价。用10-25 µg /mL ABF®预处理后，将BEAS-2B细胞暴露于LPS，并分析炎症介质（肿瘤坏死因子[TNF]-α、白细胞介素[IL]-6，IL-8，活化后调节，正常T细胞表达和推测分泌[RANTES]，IL-1β，环氧合酶-2 [考克斯-2]和诱导型一氧化氮合酶[iNOS]）的表达。在LPS刺激的BEAS-2B细胞中，ABF®预处理在10和25 µg/mL剂量下显著降低TNF-α、IL-6、IL-8、RANTES、IL-1β和考克斯-2的mRNA表达。ABF®还减弱了TNF- α、IL-6、IL-8和RANTES蛋白的分泌，如酶联免疫吸附测定所示。蛋白质印迹分析显示ABF®处理后考克斯-2和iNOS的表达降低。ABF®预处理后，ROS产生减少，细胞周期停滞在G 0/G1和S期。我们的结果表明，ABF®可能具有作为抑制气道炎症的营养药物的潜力。
To demonstrate the anti-inflammatory activity of Aronia melanocarpa fruit extract, human bronchial epithelial cells (BEAS-2B) were treated with lipopolysaccharide (LPS) and the effects of aronia bioactive fraction (ABF®), anthocyanin enriched extract from the fruit of A. melanocarpa, were evaluated. Following pretreatment with ABF® at 10–25 µg /mL, BEAS-2B cells were exposed to LPS and the expression of inflammatory mediators (tumor necrosis factor [TNF]-α, interleukin [IL]-6, IL-8, regulated upon activation, normal T cell expressed and presumably secreted [RANTES], IL-1β, cyclooxygenase-2 [COX-2], and inducible nitric oxide synthase [iNOS]) was analyzed. In LPS-stimulated BEAS-2B cells, ABF® pretreatment significantly decreased the mRNA expression of TNF-α, IL-6, IL-8, RANTES, IL-1β, and COX-2 at doses of 10 and 25 µg/mL. ABF® also attenuated the secretion of TNF- α, IL-6, IL-8, and RANTES protein, as demonstrated by enzyme linked immunosorbent assay. Western blot analyses revealed the decreased expression of COX-2 and iNOS following ABF® treatment. ROS production was decreased, and the cell cycle was arrested at the G0/G1 and S phases following ABF® pretreatment. Our results suggest that ABF® may have potential as a nutraceutical agent for the suppression of airway inflammation.
登录
查看更多内容
影响因子：
9.6
作者：
Mitchell, Patrick D.;O'Byrne, Paul M.
通讯作者：
O'Byrne, Paul M.
DOI：
10.1165/rcmb.2011-0011rt
发表时间：
2011-08-01
影响因子：
6.4
作者：
Parker, Dane;Prince, Alice
通讯作者：
Prince, Alice
影响因子：
2.8
作者：
Hendaus MA;Jomha FA;Alhammadi AH
通讯作者：
Alhammadi AH
影响因子：
3.4
作者：
Liu, Xuefang;Yin, Sugai;Zhao, Peng
通讯作者：
Zhao, Peng
影响因子：
4.4
作者：
Ohgami, K;Ilieva, I;Ohno, S
通讯作者：
Ohno, S