Complex expression pattern of Wnt ligands and frizzled receptors in human placenta and its trophoblast subtypes.
Complex expression pattern of Wnt ligands and frizzled receptors in human placenta and its trophoblast subtypes.
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DOI：
10.1016/j.placenta.2006.11.003
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发表时间：
2007-04
期刊：
影响因子：
3.8
通讯作者：
Knoefler, A.
中科院分区：
文献类型：
--
作者：
Sonderegger, S.;Husslein, H.;Leisser, C.;Knoefler, A.
由外源性和内源性Wnt配体引起的经典无翅（Wnt）信号传导最近被证明在人滋养层细胞的侵袭性分化中起关键作用。为了深入了解发育调节因子的表达模式，我们使用半定量PCR分析了人胎盘和不同滋养层模型系统中的所有人Wnt配体及其卷曲（FZD）受体。19种Wnt配体中的14种和10种FZD受体中的8种在胎盘组织中可检测到，然而，表达模式随胎龄和不同滋养层亚型之间的变化而变化，表明细胞特异性功能。除了通过经典途径起作用的Wnt配体之外，还可以鉴定非经典配体如Wnt-5a，其也可以激活替代Wnt信号传导途径或抑制经典Wnt信号传导。蛋白质印迹分析揭示了Wnt-5a从原代滋养层培养物和滋养层细胞系的分泌。为了评估Wnt-5a的潜在作用，用含有8个T细胞因子（TCF）DNA识别序列的荧光素酶报告质粒转染SGHPL-5滋养层细胞，所述DNA识别序列仅通过经典Wnt信号传导途径激活。荧光素酶分析显示，Wnt-3a诱导的报告活性被抑制重组Wnt-5a，表明在滋养层中的拮抗作用。这些数据表明，Wnt配体和FZD受体的复杂网络可以调节人胎盘的发育过程。
Canonical Wingless (Wnt) signalling provoked by exogenous and endogenous Wnt ligands was recently shown to play a crucial role in the invasive differentiation of human trophoblasts. To gain insights into the expression pattern of the developmental regulators, we analysed all human Wnt ligands and their frizzled (FZD) receptors in the human placenta and different trophoblast model systems using semi-quantitative PCR. Fourteen out of 19 Wnt ligands and 8 out of 10 FZD receptors were detectable in placental tissues, however, expression patterns varied with gestational age and between different trophoblast subtypes suggesting cell-specific functions. Besides Wnt ligands acting through the canonical pathway, non-canonical ligands such as Wnt-5a, which may also activate alternative Wnt signalling pathways or inhibit canonical Wnt signalling, could be identified. Western blot analyses revealed secretion of Wnt-5a from primary trophoblast cultures and trophoblastic cell lines. To evaluate the potential role of Wnt-5a, SGHPL-5 trophoblast cells were transfected with luciferase reporter plasmids harbouring eight T-cell factor (TCF) DNA-recognition sequences which are exclusively activated through the canonical Wnt signalling pathway. Luciferase assays revealed that Wnt-3a-induced reporter activity was repressed by recombinant Wnt-5a indicating an antagonistic role in trophoblasts. The data suggest that a complex network of Wnt ligands and FZD receptors may regulate developmental processes of the human placenta.
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影响因子：
4
作者：
Kohn, AD;Moon, RT
通讯作者：
Moon, RT
DOI：
10.1006/bbrc.2001.6076
发表时间：
2001-12-21
影响因子：
3.1
作者：
Katoh, M;Kirikoshi, H;Shiokawa, K
通讯作者：
Shiokawa, K
DOI：
10.1006/bbrc.2001.5105
发表时间：
2001-06-29
影响因子：
3.1
作者：
Saitoh, T;Hirai, M;Katoh, M
通讯作者：
Katoh, M
影响因子：
56.9
作者：
He, TC;Sparks, AB;Kinzler, KW
通讯作者：
Kinzler, KW
影响因子：
6.1
作者：
Choy, MY;Manyonda, IT
通讯作者：
Manyonda, IT