Differential adipogenic and inflammatory properties of small adipocytes in Zucker Obese and Lean rats.
Differential adipogenic and inflammatory properties of small adipocytes in Zucker Obese and Lean rats.
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DOI：
10.1177/1479164110386126
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发表时间：
2010-10
影响因子：
2.4
通讯作者：
Tsao P
中科院分区：
文献类型：
--
作者：
Liu A;Sonmez A;Yee G;Bazuine M;Arroyo M;Sherman A;McLaughlin T;Reaven G;Cushman S;Tsao P
We recently reported that a preponderance of small adipose cells, decreased expression of cell differentiation markers, and enhanced inflammatory activity in human subcutaneous whole adipose tissue were associated with insulin resistance. To test the hypothesis that small adipocytes exhibited these differential properties, we characterized small adipocytes from epididymal adipose tissue of Zucker Obese (ZO) and Lean (ZL) rats. Rat epididymal fat pads were removed and adipocytes isolated by collagenase digestion. Small adipocytes were separated by sequential filtration through nylon meshes. Adipocytes were fixed in osmium tetroxide for cell size distribution analysis via Beckman Coulter Multisizer. Quantitative real-time PCR for cell differentiation and inflammatory genes was performed. Small adipocytes represented a markedly greater percentage of the total adipocyte population in ZO than ZL rats (58±4% vs 12±3%, p<0.001). In ZO rats, small as compared to total adipocytes had 4-fold decreased adiponectin, and 4-fold increased visfatin and IL-6 levels. Comparison of small adipocytes in ZO versus ZL rats revealed 3-fold decreased adiponectin and PPARγ levels, and 2.5-fold increased IL-6. In conclusion, ZO rat adipose tissue harbors a large proportion of small adipocytes that manifest impaired cell differentiation and pro-inflammatory activity, two mechanisms by which small adipocytes may contribute to insulin resistance.
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影响因子：
2.9
作者：
Liu, Alice;McLaughlin, Tracey;Liu, Teresa;Sherman, Arthur;Yee, Gail;Abbasi, Fahim;Lamendola, Cindy;Morton, John;Cushman, Samuel W.;Reaven, Gerald M.;Tsao, Philip S.
通讯作者：
Tsao, Philip S.
影响因子：
6.9
作者：
McLaughlin, Tracey M.;Liu, T.;Yee, Gail;Abbasi, F.;Lamendola, C.;Reaven, G. M.;Tsao, P.;Cushman, S. W.;Sherman, A.
通讯作者：
Sherman, A.
DOI：
10.1016/j.ecl.2008.06.005
发表时间：
2008-09-01
影响因子：
4.5
作者：
Reaven, Gerald M.
通讯作者：
Reaven, Gerald M.
影响因子：
4.8
作者：
Gustafson, B;Smith, U
通讯作者：
Smith, U
影响因子：
--
作者：
FAUST, IM;JOHNSON, PR;HIRSCH, J
通讯作者：
HIRSCH, J