Implantation of cauda equina nerve roots through a biodegradable scaffold at the conus medullaris in rat.
Implantation of cauda equina nerve roots through a biodegradable scaffold at the conus medullaris in rat.
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DOI：
10.1016/j.spinee.2014.01.059
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发表时间：
2014-09-01
期刊：
影响因子：
--
通讯作者：
Windebank AJ
中科院分区：
文献类型：
--
作者：
Grahn PJ;Vaishya S;Knight AM;Chen BK;Schmeichel AM;Currier BL;Spinner RJ;Yaszemski MJ;Windebank AJ
发生在脊髓脊髓圆锥的创伤性损伤会对中枢神经系统和马尾神经根造成永久性损害。这项概念验证研究确定了将神经根植入可生物降解的支架是否会促进损伤后的再生。所有涉及老鼠的实验工作都是根据梅奥诊所机构动物护理和使用委员会(IACUC)批准的指南进行的。对32只SD大鼠施行外科手术。将4根马尾神经根直接或通过聚乳酸-乙醇酸(PLGA)支架重新植入脊髓腹侧残端。将这些实验组与对照组进行比较，在对照组中，神经被插入放置在脊髓和神经根之间的肌肉筋膜屏障中。动物在四周后被处死。这项工作由作者所在的机构、莫顿治愈瘫痪基金、克雷格·H·尼尔森基金会和NIBIB赠款R01 EB 02390资助。研究经费和得出的结论之间不存在利益冲突。在所有治疗组中，损伤侧脊髓吻端的运动神经元计数没有差异，这意味着植入的神经根具有相同的再生潜力。经单因素方差分析和Tukey‘s后验，PLGA支架治疗组轴突再生情况较对照组有统计学意义的改善(P<；0.05，支架n=11，对照n=11)。这项初步研究表明，PLGA支架促进了轴突向周围神经根的再生。然而，观察到的再生轴突数量有限，并未导致功能恢复。未来的实验将使用不同的支架材料和可能的生长因子或酶来增加轴突数量。
Traumatic injuries occurring at the conus medullaris of the spinal cord cause both permanent damage to the central nervous system, and to the cauda equina nerve roots. This proof of concept study determined whether implanting the nerve roots into a biodegradable scaffold would improve regeneration after injury. All experimental work involving rats was performed according to approved guidelines by the Mayo Clinic Institutional Animal Care and Use Committee (IACUC). Surgical procedures were performed on 32 Sprague Dawley rats. Four ventral cauda equina nerve roots were re-implanted either directly into the ventral cord stump or through a poly(lactic-co-glycolic acid) (PLGA) scaffold. These experimental groups were compared to a control group in which the nerves were inserted into a muscle fascia barrier that was placed between the spinal cord and nerve roots. Animals were sacrificed at four weeks. This work was funded by the authors' institution; Morton Cure Paralysis Fund; The Craig H. Neilsen Foundation; and NIBIB grant R01 EB 02390. There was no conflict of interest between the study funding and the conclusions drawn. There was no difference in motor neuron counts in the spinal cord rostral to the injury in all treatment groups, implying equal potential for regeneration into implanted nerve roots. One-way ANOVA testing, with Tukey's post-test, showed a statistically significant improvement in axon regeneration through the injury in the PLGA scaffold treatment group compared to the control (p<0.05, scaffold n=11, control n=11). This pilot study demonstrated that a PLGA scaffold improved regeneration of axons into peripheral nerve roots. However, the number of regenerating axons observed was limited and did not lead to functional recovery. Future experiments will employ a different scaffold material and possible growth factors or enzymes to increase axon populations.
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影响因子：
4.8
作者：
de Ruiter, Godard C.;Spinner, Robert J.;Windebank, Anthony J.
通讯作者：
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DOI：
10.5435/jaaos-20-02-063
发表时间：
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影响因子：
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作者：
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作者：
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影响因子：
4.1
作者：
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