Synaptic vesicle-bound pyruvate kinase can support vesicular glutamate uptake.
Synaptic vesicle-bound pyruvate kinase can support vesicular glutamate uptake.
复制标题
DOI：
10.1007/s11064-008-9833-3
复制
发表时间：
2009-05
影响因子：
4.4
通讯作者：
Ueda, Tetsufumi
中科院分区：
文献类型：
--
作者：
Ishida, Atsuhiko;Noda, Yasuko;Ueda, Tetsufumi
葡萄糖代谢对正常的脑功能是必不可少的，并且在突触传递中起着至关重要的作用。最近的证据表明，通过糖酵解局部合成的ATP，特别是通过甘油醛3-磷酸脱氢酶/3-磷酸甘油酸激酶，对突触传递至关重要。我们提出的证据表明，突触囊泡相关丙酮酸激酶产生的ATP被用来将谷氨酸转运到突触囊泡中。在磷酸烯醇式丙酮酸（PEP）和ADP存在的情况下，分离的突触囊泡掺入[3 H]谷氨酸。丙酮酸激酶激活剂和抑制剂刺激和减少PEP/ADP依赖的谷氨酸摄取，分别。在丙酮酸激酶激活剂存在下也形成膜电位。使用己糖激酶和葡萄糖的“ATP捕获”实验表明，由囊泡相关丙酮酸激酶产生的ATP比外源添加的ATP更容易使用。其他神经递质如GABA、多巴胺和5-羟色胺也以PEP/ADP依赖的方式被摄取到粗突触囊泡中。糖酵解局部产生的ATP支持囊泡积累的神经递质的可能性进行了讨论。
Glucose metabolism is essential for normal brain function and plays a vital role in synaptic transmission. Recent evidence suggests that ATP synthesized locally by glycolysis, particularly via glyceraldehyde 3-phosphate dehydrogenase/3-phosphoglycerate kinase, is critical for synaptic transmission. We present evidence that ATP generated by synaptic vesicle-associated pyruvate kinase is harnessed to transport glutamate into synaptic vesicles. Isolated synaptic vesicles incorporated [3H]glutamate in the presence of phosphoenolpyruvate (PEP) and ADP. Pyruvate kinase activators and inhibitors stimulated and reduced PEP/ADP-dependent glutamate uptake, respectively. Membrane potential was also formed in the presence of pyruvate kinase activators. “ATP-trapping” experiments using hexokinase and glucose suggest that ATP produced by vesicle-associated pyruvate kinase is more readily used than exogenously added ATP. Other neurotransmitters such as GABA, dopamine, and serotonin were also taken up into crude synaptic vesicles in a PEP/ADP-dependent manner. The possibility that ATP locally generated by glycolysis supports vesicular accumulation of neurotransmitters is discussed.
登录
查看更多内容
影响因子：
5.5
作者：
BACHELARD, HS;COX, DWG;DROWER, J
通讯作者：
DROWER, J
影响因子：
4.7
作者：
GHAJAR, JBG;PLUM, F;DUFFY, TE
通讯作者：
DUFFY, TE
影响因子：
56.9
作者：
Bellocchio, EE;Reimer, RJ;Edwards, RH
通讯作者：
Edwards, RH
影响因子：
4.7
作者：
DIRKS, B;HANKE, J;WICKOP, G
通讯作者：
WICKOP, G
影响因子：
16.2
作者：
Fremeau, RT;Troyer, MD;Edwards, RH
通讯作者：
Edwards, RH