Effect of estrogen on nitric oxide-induced relaxation of the rabbit urethra.
Effect of estrogen on nitric oxide-induced relaxation of the rabbit urethra.
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雌激素对一氧化氮诱导的兔尿道松弛的影响。
DOI：
10.1016/s0014-2999(97)01335-6
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发表时间：
1997
影响因子：
5
通讯作者：
S. Ueda
中科院分区：
文献类型：
--
作者：
W. Takahashi;M. Yoshida;Y. Wada;S. Goto;A. Inadome;M. Yono;S. Ueda
我们观察了雌激素对一氧化氮(NO)介导的兔尿道松弛的影响。雌性新西兰大白兔，4~5周龄，每周肌肉注射雌二醇二丙酸酯5 mg/kg(雌激素组)或生理盐水(对照组)，共2周。电场刺激(超最大电压，脉冲宽度2ms，频率0.3~15 Hz，S串3次)引起两组大鼠尿道条的频率依赖性松弛，这种效应可被N-硝基-L-精氨酸(Nω-Nitro-L-Arinine，L-NNA)抑制。这种抑制作用可通过加入L精氨酸而被克服。雌激素组刺激氮能神经引起的松弛明显低于对照组。硝普钠所致的尿路松弛两组间差异无统计学意义。采用微透析法测定两组大鼠尿道条中亚硝酸盐/硝酸盐(亚硝酸盐/硝酸盐/−/NO3-−)水平，以评价神经刺激过程中尿道条中NO的产生情况。雌激素组电场刺激时NO_2-−/NO_3-−生成量明显低于对照组。对照组NADPH黄递酶阳性反应强于雌激素组。结果提示，雌激素可降低兔尿道平滑肌一氧化氮合酶活性，抑制刺激氮能神经所致的松弛作用。
We evaluated the effect of estrogen on nitric oxide (NO)-mediated urethral relaxation in rabbits. Female New Zealand white rabbits, 4–5 weeks old, were treated with 5 mg/kg estradiol dipropionate (estrogen group) or saline (control group) injected intramuscularly weekly for 2 weeks. Electrical field stimulation (supramaximum voltage, 2 ms pulse duration, 0.3–15 Hz and 3 s train) caused frequency-dependent relaxation of urethral strips in both groups, which was inhibited by Nω-nitro-l-arginine (l-NNA). This inhibition was overcome by addition of l-arginine. The relaxation induced by nitrergic nerve stimulation was significantly lower in the estrogen group than in the control group. There was no significant difference in sodium nitroprusside-induced urethral relaxation between the two groups. The production of NO in urethral strips during nitrergic nerve stimulation was evaluated by measuring nitrite/nitrate (NO2−/NO3−) levels in both groups, using microdialysis. The NO2−/NO3−production during electrical field stimulation in the estrogen group was significantly less than that in the control group. The NADPH diaphorase-positive reaction in the control group was greater than that in the estrogen group. The results suggest that estrogen treatment may reduce NO synthase activity, and inhibit the relaxation induced by nitrergic nerve stimulation in rabbit urethral smooth muscle.
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有证据表明，动脉粥样硬化兔中响应内毒素的诱导型一氧化氮合酶的表达增强。
影响因子：
20.1
作者：
Sobey,CG;Brooks2nd,RM;Heistad,DD
通讯作者：
Heistad,DD
NG-硝基-L-精氨酸抑制兔尿道平滑肌的非肾上腺素能、非胆碱能松弛。
影响因子：
6.1
作者：
Dokita,S;Morgan,WR;Wheeler,MA;Yoshida,M;Latifpour,J;Weiss,RM
通讯作者：
Weiss,RM
雌激素对兔膀胱和尿道重量和毒蕈碱胆碱能受体密度的影响。
DOI：
10.1016/s0022-5347(17)45980-5
发表时间：
1986
期刊：
The Journal of urology
影响因子：
--
作者：
Shapiro,E
通讯作者：
Shapiro,E
雌激素撤退选择性地增加兔基底动脉的血清素反应性。
影响因子：
6.1
作者：
Shay,J;Futo,J;Badrov,N;Moss,J
通讯作者：
Moss,J
DOI：
10.1073/pnas.88.17.7797
发表时间：
1991-09-01
影响因子：
11.1
作者：
DAWSON, TM;BREDT, DS;SNYDER, SH
通讯作者：
SNYDER, SH