Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response.
Disease-associated mutations in a bifunctional aminoacyl-tRNA synthetase gene elicit the integrated stress response.
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DOI：
10.1016/j.jbc.2021.101203
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Musier-Forsyth K
中科院分区：
文献类型：
--
作者：
Jin D;Wek SA;Kudlapur NT;Cantara WA;Bakhtina M;Wek RC;Musier-Forsyth K
Aminoacyl-tRNA synthetases (ARSs) catalyze the charging of specific amino acids onto cognate tRNAs, an essential process for protein synthesis. Mutations in ARSs are frequently associated with a variety of human diseases. The human EPRS1 gene encodes a bifunctional glutamyl-prolyl-tRNA synthetase (EPRS) with two catalytic cores and appended domains that contribute to nontranslational functions. In this study, we report compound heterozygous mutations in EPRS1, which lead to amino acid substitutions P14R and E205G in two patients with diabetes and bone diseases. While neither mutation affects tRNA binding or association of EPRS with the multisynthetase complex, E205G in the glutamyl-tRNA synthetase (ERS) region of EPRS is defective in amino acid activation and tRNAGlu charging. The P14R mutation induces a conformational change and altered tRNA charging kinetics in vitro. We propose that the altered catalytic activity and conformational changes in the EPRS variants sensitize patient cells to stress, triggering an increased integrated stress response (ISR) that diminishes cell viability. Indeed, patient-derived cells expressing the compound heterozygous EPRS show heightened induction of the ISR, suggestive of disruptions in protein homeostasis. These results have important implications for understanding ARS-associated human disease mechanisms and development of new therapeutics.
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影响因子：
3.5
作者：
Boczonadi V;Jennings MJ;Horvath R
通讯作者：
Horvath R
影响因子：
16
作者：
Arif, Abul;Jia, Jie;Mukhopadhyay, Rupak;Willard, Belinda;Kinter, Michael;Fox, Paul L.
通讯作者：
Fox, Paul L.
影响因子：
14.8
作者：
Guo, Min;Schimmel, Paul
通讯作者：
Schimmel, Paul
影响因子：
21.3
作者：
通讯作者：
--
DOI：
10.1016/bs.enz.2020.06.009
发表时间：
2020-01-01
期刊：
BIOLOGY OF AMINOACYL-TRNA SYNTHETASES
影响因子：
--
作者：
Jiang, Lei;Jones, Julia;Yang, Xiang-Lei
通讯作者：
Yang, Xiang-Lei