Study on immobilization of marine oil-degrading bacteria by carrier of algae materials
Study on immobilization of marine oil-degrading bacteria by carrier of algae materials
复制标题
藻类材料载体固定化海洋石油降解菌的研究
DOI：
10.1007/s11274-018-2438-3
复制
发表时间：
2018-05
影响因子：
4.1
通讯作者：
Zheng Li
中科院分区：
文献类型：
--
作者：
Zhang Yiran;Gao Wei;Lin Faxiang;Han Bin;He Changfei;Li Qian;Gao Xiangxing;Cui Zhisong;Sun Chengjun;Zheng Li
研究了浒苔渣和海带渣两种藻类材料对细菌的固定化作用。通过柴油吸收率比较了它们的亲油性。芽孢杆菌的固定化效率。E3的测量，以评估这些载体是否满足生物降解的石油泄漏的要求。采用无菌载体和非无菌载体吸附固定化细菌，比较两种处理的差异。采用重量法和GC-MS法测定油的降解率。结果表明，在模拟海水体系中，浒苔残渣和海带残渣对柴油的吸附率分别为411%和273%，经2 h冲刷后，吸附率分别为105%和120%。固定化芽孢杆菌后，E3，降解21 d后，油脂降解率均在65%以上。GC-MS分析表明，两种固定化菌对总烷烃和总多环芳烃的降解率均高于70%，而游离菌对总烷烃和总多环芳烃的降解率分别为63%和66%。载体固定化细菌比自由细菌降解更多的高分子量烷烃和高分子量多环芳烃。未灭菌海带渣固定化细菌的降解率明显高于灭菌海带渣固定化细菌。利用海带渣制备固定化细胞具有较高的细胞吸附率，且制备成本低、效率高。实验表明，两种藻类材料，特别是海带残渣，在海洋溢油生物修复中具有潜在的应用价值。
This study investigated the immobilizations with of bacteria two kinds of algal materials,Enteromorpharesidue and kelp residue. The lipophilicity of them were compared by diesel absorption rates. The immobilization efficiency ofBacillussp. E3 was measured to evaluate whether these carriers would satisfy the requirement for biodegradation of oil spills. The bacteria were immobilized through adsorption with the sterilized and non-sterilized carriers to compare the differences between the two treatments. Oil degradation rates were determined using gravimetric and GC–MS methods. Results showed the absorption rates ofEnteromorpharesidue and kelp residue for diesel were 411 and 273% respectively and remained approximately 105 and 120% after 2 h of erosion in simulated seawater system. After immobilized ofBacillussp. E3, the oil degradation rates of them were higher than 65% after 21 days biodegradations. GC–MS analysis showed that two immobilizations degraded higher than 70% of the total alkane and the total PAHs, whereas the free bacteria degraded 63% of the total alkane and 66% the total PAHs. And the bacteria immobilized with the carriers degraded more HMW–alkanes and HMW-PAHs than the free bacteria. The bacteria immobilized by non-sterilized kelp residue showed a considerably higher degradation rate than that using sterilized kelp residue. A considerably higher cells absorption rate of immobilization was obtained when using kelp residue, and the preparation of immobilization was low cost and highly efficient. The experiments show the two algae materials, especially the kelp residue, present potential application in bioremediation of marine oil spills.
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DOI：
10.1515/botm.1979.22.4.237
发表时间：
1979
期刊：
--
影响因子：
--
作者：
B. Velimirov
通讯作者：
B. Velimirov
DOI：
10.2991/icseee-15.2016.69
发表时间：
2016-04
期刊：
--
影响因子：
--
作者：
Y. Ji;Haomeng Xu;Xiaojie Wang
通讯作者：
Y. Ji;Haomeng Xu;Xiaojie Wang
影响因子：
1.4
作者：
C. Lei
通讯作者：
C. Lei
DOI：
--
发表时间：
2011
期刊：
Materials review
影响因子：
--
作者：
Yongzhong Wang
通讯作者：
Yongzhong Wang
DOI：
10.3844/ajessp.2011.423.440
发表时间：
2011-11
期刊：
American Journal of Environmental Sciences
影响因子：
--
作者：
D. Dave;A. Ghaly
通讯作者：
D. Dave;A. Ghaly