Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1 and profilin in C. elegans.
Sarcomeric actin organization is synergistically promoted by tropomodulin, ADF/cofilin, AIP1 and profilin in C. elegans.
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DOI：
10.1242/jcs.040477
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发表时间：
2008-12-01
影响因子：
4
通讯作者：
Ono S
中科院分区：
文献类型：
--
作者：
Yamashiro S;Cox EA;Baillie DL;Hardin JD;Ono S
横纹肌中粗细纤维的组织对于有效产生收缩力是重要的。肌动蛋白丝的长度是一致的，并有规律地排列成条纹状。原调蛋白位于肌动蛋白丝的尖端，是肌节组装的关键调节器。在这里，我们报告了原调节蛋白与秀丽隐杆线虫横纹肌肌动蛋白丝动力学增强剂的意想不到的协同功能。原调蛋白的尖端封顶抑制ADF/cofilin体外解聚肌动蛋白丝。然而，在体内，原调节蛋白的消耗强烈地增强了ADF/cofilin突变体中肌动蛋白丝的破坏，而不是拮抗性地抑制表型。在AIP1和profilin突变背景中也观察到类似的原调节蛋白缺失导致的表型增强。这些体内效应不能简单地用原调节蛋白和ADF/cofilin在体外的拮抗作用来解释。因此，我们提出了一个模型，其中原调蛋白和肌动蛋白动力学增强剂协同调节肌动蛋白丝在尖端的伸长和缩短。
Sarcomeric organization of thin and thick filaments in striated muscle is important for efficient generation of contractile forces. Sarcomeric actin filaments are uniform in their lengths and regularly arranged in a striated pattern. Tropomodulin caps the pointed end of actin filaments and is a critical regulator of sarcomere assembly. Here, we report unexpected synergistic functions of tropomodulin with enhancers of actin filament dynamics in Caenorhabditis elegans striated muscle. Pointed-end capping by tropomodulin inhibited actin filament depolymerization by ADF/cofilin in vitro. However, in vivo, depletion of tropomodulin strongly enhanced disorganization of sarcomeric actin filaments in ADF/cofilin mutants, rather than antagonistically suppressing the phenotype. Similar phenotypic enhancements by tropomodulin depletion were also observed in mutant backgrounds for AIP1 and profilin. These in vivo effects cannot be simply explained by antagonistic effects of tropomodulin and ADF/cofilin in vitro. Thus, we propose a model in which tropomodulin and enhancers of actin dynamics synergistically regulate elongation and shortening of actin filaments at the pointed end.
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DOI：
10.1083/jcb.101.4.1532
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Francis GR;Waterston RH
通讯作者：
Waterston RH
影响因子：
4.8
作者：
Blanchoin, L;Pollard, TD
通讯作者：
Pollard, TD
影响因子：
64.5
作者：
MILLER, DM;ORTIZ, I;EPSTEIN, HF
通讯作者：
EPSTEIN, HF
影响因子：
4.8
作者：
Laing, Nigel G.
通讯作者：
Laing, Nigel G.
影响因子：
3.5
作者：
LABEIT, S;GIBSON, T;TRINICK, J
通讯作者：
TRINICK, J