Brahma is required for proper expression of the floral repressor FLC in Arabidopsis.
Brahma is required for proper expression of the floral repressor FLC in Arabidopsis.
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DOI：
10.1371/journal.pone.0017997
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发表时间：
2011-03-21
期刊：
影响因子：
3.7
通讯作者：
Reyes JC
中科院分区：
文献类型：
--
作者：
Farrona S;Hurtado L;March-Díaz R;Schmitz RJ;Florencio FJ;Turck F;Amasino RM;Reyes JC
BRAHMA（BRM）是拟南芥中SWI/SNF染色质重塑复合物的ATP酶家族的成员。BRM以前已被证明是至关重要的营养和生殖发育。我们对brm突变体植物的开花表型进行了详细的分析，结果表明，除了抑制开花促进基因CONSTANS（CO）、FLOWERLOCUS T（FT）和CO 1过表达抑制因子（SOC 1）外，BRM还抑制一般开花抑制因子FLOWERLOCUS C（FLC）的表达。因此，在brm突变体植物中，FLC表达升高，并且FLC染色质表现出组蛋白H3赖氨酸4三甲基化水平升高和H3赖氨酸27三甲基化水平降低，表明BRM在FLC基因座处施加抑制性染色质构型。然而，brm突变体显示正常的春化反应，表明BRM不参与春化介导的FLC阻遏。双突变体的分析表明，BRM是部分冗余的自主途径。BRM和组蛋白H2A.Z沉积机制之间的遗传相互作用的分析表明，brm突变克服了FLC活化对H2A.Z的要求，表明在不存在BRM的情况下，组成性开放的染色质构象使H2A.Z失活。BRM在拟南芥的相位转换中是至关重要的。因此，BRM抑制开花促进基因CO、FT和SOC 1以及开花抑制因子FLC的表达。我们的研究结果表明，BRM控制FLC的表达，通过创建一个抑制染色质配置的基因座。
BRAHMA (BRM) is a member of a family of ATPases of the SWI/SNF chromatin remodeling complexes from Arabidopsis. BRM has been previously shown to be crucial for vegetative and reproductive development. Here we carry out a detailed analysis of the flowering phenotype of brm mutant plants which reveals that, in addition to repressing the flowering promoting genes CONSTANS (CO), FLOWERING LOCUS T (FT) and SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1), BRM also represses expression of the general flowering repressor FLOWERING LOCUS C (FLC). Thus, in brm mutant plants FLC expression is elevated, and FLC chromatin exhibits increased levels of histone H3 lysine 4 tri-methylation and decreased levels of H3 lysine 27 tri-methylation, indicating that BRM imposes a repressive chromatin configuration at the FLC locus. However, brm mutants display a normal vernalization response, indicating that BRM is not involved in vernalization-mediated FLC repression. Analysis of double mutants suggests that BRM is partially redundant with the autonomous pathway. Analysis of genetic interactions between BRM and the histone H2A.Z deposition machinery demonstrates that brm mutations overcome a requirement of H2A.Z for FLC activation suggesting that in the absence of BRM, a constitutively open chromatin conformation renders H2A.Z dispensable. BRM is critical for phase transition in Arabidopsis. Thus, BRM represses expression of the flowering promoting genes CO, FT and SOC1 and of the flowering repressor FLC. Our results indicate that BRM controls expression of FLC by creating a repressive chromatin configuration of the locus.
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