Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus.
Genome-Wide Identification and Characterization of NODULE-INCEPTION-Like Protein (NLP) Family Genes in Brassica napus.
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甘蓝型油菜中结节起始样蛋白 (NLP) 家族基因的全基因组鉴定和表征
DOI：
10.3390/ijms19082270
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发表时间：
2018-08-02
影响因子：
5.6
通讯作者：
Lu K
中科院分区：
文献类型：
--
作者：
Liu M;Chang W;Fan Y;Sun W;Qu C;Zhang K;Liu L;Xu X;Tang Z;Li J;Lu K
nlp是一种保守的植物特异性转录因子，在植物对氮缺乏的反应中起着至关重要的作用。然而，甘蓝型油菜NLP家族基因的进化关系和特征尚不清楚。本研究共鉴定出31个NLP基因，其中16个位于A亚基因组，15个位于C亚基因组。亚细胞定位预测表明，大多数BnaNLP蛋白定位于细胞核。系统发育分析表明，NLP基因家族可分为3个类群，单子叶和双子叶的祖先在分化之前至少存在3个NLP基因的古老拷贝。III群NLP基因的祖先可能在芸苔科物种中经历了不止一次的重复。三维结构分析表明，BnaNLP7-1蛋白中有14个氨基酸参与DNA结合，而BnaNLP7-1蛋白中保守的两个RWP-RK和PB1结构域未发现结合位点。表达谱分析表明，BnaNLP基因在大多数器官中表达，但倾向于在单个器官中高表达。例如，BnaNLP6亚家族成员主要在根中表达，而4个BnaNLP7亚家族成员在叶片中高度表达。BnaNLP基因在缺氮条件下也表现出不同的表达模式。缺氮条件下，BnaNLP1/4/5/9亚家族成员均表达上调，BnaNLP2/6亚家族成员表达下调，BnaNLP7/8亚家族成员在不同器官中表达模式不同。这些结果提供了甘蓝型油菜NLP基因的全面进化历史，以及BnaNLP基因在氮素缺乏反应中的生物学功能。
NODULE-INCEPTION-like proteins (NLPs) are conserved, plant-specific transcription factors that play crucial roles in responses to nitrogen deficiency. However, the evolutionary relationships and characteristics of NLP family genes in Brassica napus are unclear. In this study, we identified 31 NLP genes in B. napus, including 16 genes located in the A subgenome and 15 in the C subgenome. Subcellular localization predictions indicated that most BnaNLP proteins are localized to the nucleus. Phylogenetic analysis suggested that the NLP gene family could be divided into three groups and that at least three ancient copies of NLP genes existed in the ancestor of both monocots and dicots prior to their divergence. The ancestor of group III NLP genes may have experienced duplication more than once in the Brassicaceae species. Three-dimensional structural analysis suggested that 14 amino acids in BnaNLP7-1 protein are involved in DNA binding, whereas no binding sites were identified in the two RWP-RK and PB1 domains conserved in BnaNLP proteins. Expression profile analysis indicated that BnaNLP genes are expressed in most organs but tend to be highly expressed in a single organ. For example, BnaNLP6 subfamily members are primarily expressed in roots, while the four BnaNLP7 subfamily members are highly expressed in leaves. BnaNLP genes also showed different expression patterns in response to nitrogen-deficient conditions. Under nitrogen deficiency, all members of the BnaNLP1/4/5/9 subfamilies were upregulated, all BnaNLP2/6 subfamily members were downregulated, and BnaNLP7/8 subfamily members showed various expression patterns in different organs. These results provide a comprehensive evolutionary history of NLP genes in B. napus, and insight into the biological functions of BnaNLP genes in response to nitrogen deficiency.
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作者：
Blum T;Briesemeister S;Kohlbacher O
通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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