Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology.
Smooth muscle-like tissue constructs with circumferentially oriented cells formed by the cell fiber technology.
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DOI：
10.1371/journal.pone.0119010
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Takeuchi S
中科院分区：
文献类型：
--
作者：
Hsiao AY;Okitsu T;Onoe H;Kiyosawa M;Teramae H;Iwanaga S;Kazama T;Matsumoto T;Takeuchi S
许多含有平滑肌的器官和组织的正常功能在很大程度上取决于平滑肌细胞在适当方向上的复杂组织。因此，控制三维细胞结构中的细胞取向是平滑肌工程组织中的一个重要问题。然而，在微观尺度上精确控制细胞方向的能力不能通过各种常用的3D组织工程构建块（如球体）来实现。本文介绍了由去分化脂肪（DFAT）细胞分化而成的具有周向平滑肌样细胞的螺旋形三维细胞结构的形成。通过细胞纤维技术，将DFAT细胞悬浮在具有优化刚度的细胞外蛋白混合物中，包裹在海藻酸盐壳微纤维的核心区域，并均匀地向纵向排列。在分化诱导到平滑肌谱系后，DFAT细胞纤维自组装成卷曲的弹簧结构，细胞变得周向。通过改变芯壳微纤维的初始直径，我们证明了弹簧的节距和直径是可以控制的。诱导分化21 d后，细胞纤维中asma阳性细胞和calponin阳性细胞的比例较高。我们的技术创造了这些平滑肌状的弹簧结构，可以在3D中精确控制细胞的排列和方向。这些结构可以进一步作为组织工程的构建块，用于临床治疗的更大的器官和细胞植入物。
The proper functioning of many organs and tissues containing smooth muscles greatly depends on the intricate organization of the smooth muscle cells oriented in appropriate directions. Consequently controlling the cellular orientation in three-dimensional (3D) cellular constructs is an important issue in engineering tissues of smooth muscles. However, the ability to precisely control the cellular orientation at the microscale cannot be achieved by various commonly used 3D tissue engineering building blocks such as spheroids. This paper presents the formation of coiled spring-shaped 3D cellular constructs containing circumferentially oriented smooth muscle-like cells differentiated from dedifferentiated fat (DFAT) cells. By using the cell fiber technology, DFAT cells suspended in a mixture of extracellular proteins possessing an optimized stiffness were encapsulated in the core region of alginate shell microfibers and uniformly aligned to the longitudinal direction. Upon differentiation induction to the smooth muscle lineage, DFAT cell fibers self-assembled to coiled spring structures where the cells became circumferentially oriented. By changing the initial core-shell microfiber diameter, we demonstrated that the spring pitch and diameter could be controlled. 21 days after differentiation induction, the cell fibers contained high percentages of ASMA-positive and calponin-positive cells. Our technology to create these smooth muscle-like spring constructs enabled precise control of cellular alignment and orientation in 3D. These constructs can further serve as tissue engineering building blocks for larger organs and cellular implants used in clinical treatments.
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影响因子：
14
作者：
Norotte, Cyrille;Marga, Francois S.;Niklason, Laura E.;Forgacs, Gabor
通讯作者：
Forgacs, Gabor
影响因子：
--
作者：
Shen, Jin Ye;Chan-Park, Mary B.;Chan, Vincent
通讯作者：
Chan, Vincent
影响因子：
41.2
作者：
Onoe, Hiroaki;Okitsu, Teru;Takeuchi, Shoji
通讯作者：
Takeuchi, Shoji
影响因子：
82.9
作者：
Cleaver, O;Melton, DA
通讯作者：
Melton, DA
DOI：
10.1073/pnas.0604850103
发表时间：
2006-08-08
影响因子：
11.1
作者：
Rodriguez, Larissa V.;Alfonso, Zeni;Ignarro, Louis J.
通讯作者：
Ignarro, Louis J.