Etched glass microarrays with differential resonance for enhanced contrast and sensitivity of surface plasmon resonance imaging analysis.
Etched glass microarrays with differential resonance for enhanced contrast and sensitivity of surface plasmon resonance imaging analysis.
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DOI：
10.1021/ac200881q
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发表时间：
2011-08-01
影响因子：
7.4
通讯作者：
Cheng, Quan
中科院分区：
文献类型：
--
作者：
Linman, Matthew J.;Abbas, Abdennour;Roberts, Christopher C.;Cheng, Quan
我们报告了表面等离子体共振成像（SPRI）分析的金涂层蚀刻玻璃阵列底物的制造和表征，其性能显着增强，尤其是图像对比度和灵敏度。在蚀刻井和周围区域之间的谐振角度，导致阵列点共振，并显着降低了背景信号显示较大斑点的阵列和最小的斑点间距产生理想的差分谐振条件，通过实验结果验证了蚀刻井的蚀刻井结构，与标准的平面金芯片相比，蚀刻井结构增强了SPR电场强度基于图像强度差异，在10-4 RIU级别上获得了蚀刻阵列的大量敏感性。允许通过蚀刻阵列芯片易于区分的微阵列成像分析获得5 nm处的霍乱毒素（CT），这使该芯片成为有史以来报告的最敏感的SPR成像底物之一结合后的放大方案。 ，以方便和明显的表面灵敏度为高通量SPR成像检测打开新的途径。
We report the fabrication and characterization of gold-coated etched glass array substrates for surface plasmon resonance imaging (SPRi) analysis with significantly enhanced performances, in particular image contrast and sensitivity. The etching of the glass substrate induces a variation in the resonance condition and thus in the resonance angle between the etched wells and the surrounding area, leading to the isolation of the array spot resonance with a significant reduction of the background signal. FDTD simulations show arrays with large spots and minimal spot-to-spot spacing yield ideal differential resonance conditions, which are verified by experimental results. Simulations also indicate the etched well structure exhibits enhanced SPR electric field intensity by three-fold as compared to standard planar gold chips. Changes in the bulk sensitivity of the etched arrays have been obtained at the 10−4 RIU level based on image intensity difference. The strong image contrast allows for improved microarray imaging analysis with easily distinguished signals from background resonance. The etched array chips are demonstrated for SPRi detection of bacterial toxins through the coating of an ultrathin SiO2 film for direct vesicle fusion that establishes a supported membrane-based biosensing interface. Protein detection with cholera toxin (CT) at 5 nM is obtained, making this chip one of the most sensitive SPR imaging substrates ever reported without a post-binding amplification scheme. Furthermore, the surface can be regenerated by Triton X-100 for repeated cycles of membrane formation, protein binding, and biomolecular removal. The reusability and enhanced performance of the etched glass array chips should find a broad range of applications, opening up new avenues for high-throughput SPR imaging detection with convenience and marked surface sensitivity.
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影响因子：
7.4
作者：
Abbas, Abdennour;Linman, Matthew J.;Cheng, Quan
通讯作者：
Cheng, Quan
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DOI：
10.3390/s90100645
发表时间：
2009
期刊：
Sensors (Basel, Switzerland)
影响因子：
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作者：
Charles PT;Stubbs VR;Soto CM;Martin BD;White BJ;Taitt CR
通讯作者：
Taitt CR
影响因子：
3.9
作者：
Jung, Jaeyeon;Yuk, Jong Seol;Hyun, Jinho
通讯作者：
Hyun, Jinho
影响因子：
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作者：
Ganesan, Ramakrishnan;Kratz, Karl;Lendlein, Andreas
通讯作者：
Lendlein, Andreas
有限糖蛋白样品的表面等离子共振成像
影响因子：
4.2
作者：
Liu, Wei;Chen, Yi;Yan, Mingdi
通讯作者：
Yan, Mingdi