Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis.
Subcellular proteomic characterization of the high-temperature stress response of the cyanobacterium Spirulina platensis.
复制标题
DOI：
10.1186/1477-5956-7-33
复制
发表时间：
2009-09-02
期刊：
影响因子：
2
通讯作者：
Tanticharoen M
中科院分区：
文献类型：
--
作者：
Hongsthong A;Sirijuntarut M;Yutthanasirikul R;Senachak J;Kurdrid P;Cheevadhanarak S;Tanticharoen M
本研究从亚细胞水平分析了高温对钝顶螺旋藻蛋白质表达的影响。此外，还分析了部分差异表达蛋白的转录表达水平、表达模式聚类以及蛋白质间相互作用网络。从差异表达分析获得的结果揭示了参与双组分反应系统、DNA损伤和修复系统、分子伴侣、已知的应激相关蛋白以及参与其他生物学过程（如胶囊形成和不饱和脂肪酸生物合成）的蛋白的上调。三个细胞区室中所有差异表达蛋白的聚类显示：（i）所有组分中的大部分蛋白质是持续耐受蛋白，表明这些蛋白质在高温胁迫耐受性中的作用，（ii）光合膜中的抗性蛋白质水平比其他两个组分中的水平高2倍，这与光合系统响应高温而迅速失活有关。PPI网络分析清楚地显示了三个细胞区室之间通过蛋白质-蛋白质相互作用进行的亚细胞通讯。此外，该分析还表明温度胁迫与氮和氨同化之间的联系。
The present study examined the changes in protein expression in Spirulina platensis upon exposure to high temperature, with the changes in expression analyzed at the subcellular level. In addition, the transcriptional expression level of some differentially expressed proteins, the expression pattern clustering, and the protein-protein interaction network were analyzed. The results obtained from differential expression analysis revealed up-regulation of proteins involved in two-component response systems, DNA damage and repair systems, molecular chaperones, known stress-related proteins, and proteins involved in other biological processes, such as capsule formation and unsaturated fatty acid biosynthesis. The clustering of all differentially expressed proteins in the three cellular compartments showed: (i) the majority of the proteins in all fractions were sustained tolerance proteins, suggesting the roles of these proteins in the tolerance to high temperature stress, (ii) the level of resistance proteins in the photosynthetic membrane was 2-fold higher than the level in two other fractions, correlating with the rapid inactivation of the photosynthetic system in response to high temperature. Subcellular communication among the three cellular compartments via protein-protein interactions was clearly shown by the PPI network analysis. Furthermore, this analysis also showed a connection between temperature stress and nitrogen and ammonia assimilation.
登录
查看更多内容
影响因子：
14.9
作者：
Owttrim GW
通讯作者：
Owttrim GW
影响因子：
4.3
作者：
Allakhverdiev, Suleyman I.;Los, Dmitry A.;Murata, Norio
通讯作者：
Murata, Norio
影响因子：
5.1
作者：
Drzymalla, C;Schroda, M;Beck, CF
通讯作者：
Beck, CF
影响因子：
2.6
作者：
Fang, F;Barnum, SR
通讯作者：
Barnum, SR
影响因子：
2.1
作者：
Jeamton, Wattana;Mungpakdee, Sutada;Hongsthong, Apiradee
通讯作者：
Hongsthong, Apiradee