Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.

Fundamentals and application of magnetic particles in cell isolation and enrichment: a review.
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磁颗粒在细胞分离和富集中的基本原理和应用:综述。

DOI:
10.1088/0034-4885/78/1/016601
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发表时间:
2015-01
期刊:
Reports on progress in physics. Physical Society (Great Britain)
影响因子:
--
通讯作者:
Lewis LH
Lewis LH
中科院分区:
其他
文献类型:
--
作者:
Plouffe BD;Murthy SK;Lewis LH

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使用磁珠的磁性分选已经成为从生物悬浮液中分离关键细胞群的常规方法。由于磁体在远距离提供力的固有能力,磁性细胞操作现在是组织工程、医学和基础生物学研究中的许多过程中的标准化过程步骤。在此,我们回顾了磁性颗粒的现状,使细胞的隔离和分离,重点关注的基本管理的物理现象,性质和磁性颗粒的合成和基于磁性的细胞分离在实验室和临床环境中的当前应用。我们强调了细胞分离对生物医学研究和医学的贡献,并详细介绍了现代细胞分离方法(磁性和非磁性)。除了对基于磁性颗粒的细胞分选的当前最新技术进行回顾外,我们还讨论了基于磁性颗粒的细胞分离系统的进一步研究、开发和商业化所面临的挑战和可用的机会。
Magnetic sorting using magnetic beads has become a routine methodology for the separation of key cell populations from biological suspensions. Due to the inherent ability of magnets to provide forces at a distance, magnetic cell manipulation is now a standardized process step in numerous processes in tissue engineering, medicine, and in fundamental biological research. Herein we review the current status of magnetic particles to enable isolation and separation of cells, with a strong focus on the fundamental governing physical phenomena, properties and syntheses of magnetic particles and on current applications of magnet-based cell separation in laboratory and clinical settings. We highlight the contribution of cell separation to biomedical research and medicine and detail modern cell separation methods (both magnetic and non-magnetic). In addition to a review of the current state-of-the-art in magnet-based cell sorting, we discuss current challenges and available opportunities for further research, development and commercialization of magnetic particle-based cell separation systems.
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