A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers.
A multidisciplinary approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: a focus on potential salivary biomarkers.
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DOI：
10.1186/1479-5876-11-243
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发表时间：
2013-10-02
影响因子：
7.4
通讯作者：
Bazzichi L
中科院分区：
文献类型：
--
作者：
Ciregia F;Giusti L;Da Valle Y;Donadio E;Consensi A;Giacomelli C;Sernissi F;Scarpellini P;Maggi F;Lucacchini A;Bazzichi L
慢性疲劳综合征（CFS）是一种严重的全身性疾病，其特征是持续的、使人衰弱的和医学上无法解释的疲劳。CFS的病因和病理生理学仍然不清楚，诊断是通过患者的病史和排除其他医疗原因制定的。因此，CFS的生物标志物的可用性可用于临床研究。在本研究中，我们使用蛋白质组学方法来评估一对CFS不一致的单卵双胞胎唾液特征的整体变化。目的是评估CFS患者与其健康双胞胎唾液蛋白表达的差异。唾液样本进行双向电泳（2DE）。用Sypro对凝胶进行染色，并通过软件Progenesis Same Spot进行CFS受试者和健康受试者之间的比较，包括方差分析（ANOVA检验）。通过纳米液相色谱电喷雾串联质谱法鉴定了斑点数量变化≥2倍且p<0.05的蛋白质斑点。为了验证5种蛋白质（14-3-3蛋白ζ/δ、亲环蛋白A、胱抑素-C、蛋白S100-A7和锌-α-2-糖蛋白）的2DE发现的表达变化，我们使用了蛋白质印迹分析。此外，差异表达的蛋白质的功能进行了分析，使用的Incubity途径分析软件，目的是确定主要的经典途径和相互作用网络。蛋白质谱的分析使我们能够找到13个蛋白质在CFS中与对照相比具有不同表达。CFS中9个点表达上调，4个点表达下调。这些蛋白质属于不同的功能类别，如炎症反应，免疫系统和代谢。特别是，如通路分析所示，用我们的蛋白质构建的网络突出了炎症反应在CFS发病机制中的参与。这项研究表明，存在差异表达的蛋白质在唾液中的一对单卵双胞胎不一致的CFS，可能与疾病。因此，我们相信蛋白质组学方法可以用于定义一组潜在的诊断生物标志物，并为理解CFS的发病途径提供新的线索。
Chronic Fatigue Syndrome (CFS) is a severe, systemic illness characterized by persistent, debilitating and medically unexplained fatigue. The etiology and pathophysiology of CFS remains obscure, and diagnosis is formulated through the patient’s history and exclusion of other medical causes. Thereby, the availability of biomarkers for CFS could be useful for clinical research. In the present study, we used a proteomic approach to evaluate the global changes in the salivary profile in a couple of monozygotic twins who were discordant for CFS. The aim was to evaluate differences of salivary protein expression in the CFS patient in respect to his healthy twin. Saliva samples were submitted to two-dimensional electrophoresis (2DE). The gels were stained with Sypro, and a comparison between CFS subject and the healthy one was performed by the software Progenesis Same Spot including the Analysis of variance (ANOVA test). The proteins spot found with a ≥2-fold spot quantity change and p<0.05 were identified by Nano-liquid chromatography electrospray ionization tandem mass spectrometry. To validate the expression changes found with 2DE of 5 proteins (14-3-3 protein zeta/delta, cyclophilin A, Cystatin-C, Protein S100-A7, and zinc-alpha-2-glycoprotein), we used the western blot analysis. Moreover, proteins differentially expressed were functionally analyzed using the Ingenuity Pathways Analysis software with the aim to determine the predominant canonical pathways and the interaction network involved. The analysis of the protein profiles allowed us to find 13 proteins with a different expression in CFS in respect to control. Nine spots were up-regulated in CFS and 4 down-regulated. These proteins belong to different functional classes, such as inflammatory response, immune system and metabolism. In particular, as shown by the pathway analysis, the network built with our proteins highlights the involvement of inflammatory response in CFS pathogenesis. This study shows the presence of differentially expressed proteins in the saliva of the couple of monozygotic twins discordant for CFS, probably related to the disease. Consequently, we believe the proteomic approach could be useful both to define a panel of potential diagnostic biomarkers and to shed new light on the comprehension of the pathogenetic pathways of CFS.
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影响因子：
--
作者：
Giusti, Laura;Iacconi, Pietro;Lucacchini, Antonio
通讯作者：
Lucacchini, Antonio
影响因子：
5.6
作者：
Fábián TK;Hermann P;Beck A;Fejérdy P;Fábián G
通讯作者：
Fábián G
影响因子：
3
作者：
Eckardt, Kristin;Schober, Annette;Eckel, Juergen
通讯作者：
Eckel, Juergen
影响因子：
6.4
作者：
Alter HJ;Mikovits JA;Switzer WM;Ruscetti FW;Lo SC;Klimas N;Komaroff AL;Montoya JG;Bateman L;Levine S;Peterson D;Levin B;Hanson MR;Genfi A;Bhat M;Zheng H;Wang R;Li B;Hung GC;Lee LL;Sameroff S;Heneine W;Coffin J;Hornig M;Lipkin WI
通讯作者：
Lipkin WI
影响因子：
7.4
作者：
Baldini C;Giusti L;Ciregia F;Da Valle Y;Giacomelli C;Donadio E;Ferro F;Galimberti S;Donati V;Bazzichi L;Bombardieri S;Lucacchini A
通讯作者：
Lucacchini A