Molecularly Imprinted Photonic Crystals Based on Fusaric Acid for the Detection of Banana Fusarium Wilt
Molecularly Imprinted Photonic Crystals Based on Fusaric Acid for the Detection of Banana Fusarium Wilt
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基于镰刀菌酸的分子印迹光子晶体用于检测香蕉枯萎病
DOI：
10.1021/acsapm.1c01032
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发表时间：
2021-10
影响因子：
5
通讯作者：
Anqiang Zhang
中科院分区：
文献类型：
--
作者：
Yaling Lin;Xixiang Feng;Wei Zhang;Rui Li;Anqiang Zhang
香蕉枯萎病是由尖孢镰刀菌引起的土传病害。sp. cubense（Foc.）尖孢菌属香蕉黑胫病菌4号（Foc 4）可侵染所有香蕉品种，给香蕉种植业造成了严重的危害。农业生产中防治香蕉枯萎病最重要的手段是控制病原菌的传播和入侵。其中，对香蕉枯萎病的田间检测和鉴定是必不可少的，也是防治的基础。由于镰刀菌酸（FA）是枯萎病病原菌代谢过程中的特异产物之一，将FA与分子印迹光子晶体（MIPC）技术相结合，制备了一系列基于单分散二氧化硅、FA和交联聚合物的MIPC，设计并制备了可回收的MIPC-FA。结果表明，MIPC-FA的响应受聚合物基体的交联度和pH值的影响。结果表明，MIPC-FA能有效地检测出微量FA溶液、Foc 4培养基和Foc 4侵染的香蕉植株中的FA，且检测结果与FA浓度有关，是一种快速、有效的田间检测方法。
Banana Fusarium wilt is a soil-borne disease caused byFusarium oxysporumf. sp.cubense(Foc) in whichF. oxysporumf. sp.cubenserace 4 (Foc4) could infect all kinds of banana and has caused great damage to the banana planting industry. The most important means to prevent and control banana Fusarium wilt in agricultural production is to control the spread and invasion of pathogens. Among them, the detection and determination of banana Fusarium wilt in the field is essential and thus is the basis of protection. Since fusaric acid (FA) is one of the specific products present during the metabolic processing of Fusarium wilt pathogens, by combining FA with molecularly imprinted photonic crystal (MIPC) technologies, a series of MIPCs based on monodispersed silica, FA, and cross-linked polymers, i.e., MIPC-FA, which could be recycled, were designed and prepared. It was found that the responses of MIPC-FA were affected by the cross-linking degree of the polymer matrix and the pH values. The results showed that MIPC-FA could effectively detect FA in a solution of FA in a minute scale, Foc4-containing medium, and banana plants infected by Foc4, and the response was related to the FA concentration, which makes it an effective and fast method to detect and determine banana Fusarium wilt caused by Foc4 in the field.
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