Generation of SV40-transformed rabbit tracheal-epithelial-cell-derived blastocyst by somatic cell nuclear transfer.
Generation of SV40-transformed rabbit tracheal-epithelial-cell-derived blastocyst by somatic cell nuclear transfer.
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DOI：
10.1007/s00441-011-1296-1
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发表时间：
2012-02
影响因子：
3.6
通讯作者：
Gruenert, D. C.
中科院分区：
文献类型：
--
作者：
de Semir, D.;Maurisse, R.;Du, F.;Xu, J.;Yang, X.;Illek, B.;Gruenert, D. C.
关键词：
通过体细胞核移植（SCNT）开发大型动物模型来研究囊性纤维化（CF）等遗传性疾病的前景为增强我们对疾病病理学的理解和确定新疗法开辟了新的机会。因此，开发物种特异性体外细胞系统至关重要，这将为了解与疾病病理学相关的器官和细胞类型特异性功能提供更广泛的了解。已经进行了研究以建立转化的兔气道上皮细胞系，其表现出初级气道上皮的分化特征。本研究描述了两种 SV40 转化的兔气管上皮细胞系的成功建立和表征。这些细胞系 5RTEo- 和 9RTEo- 表达 CF 跨膜电导调节因子 (CFTR) 基因，保留上皮特异性分化形态，并显示出基于 CFTR 的 cAMP 依赖性 Cl− 离子跨汇合单层顶膜的转运。免疫细胞化学分析表明，经过多代后，9RTEo 细胞系中存在气道细胞角蛋白和紧密连接蛋白。然而，在至少 100 代后，紧密连接在两种细胞系中的功效似乎都会减弱。对 9RTEo 细胞的初步 SCNT 研究表明，SV40 转化的兔气道上皮供体细胞可用于生成囊胚。这些细胞系统为研究 CFTR 基因表达的发育和代谢调节以及兔气道上皮细胞生物学提供了有价值的模型。
The prospect of developing large animal models for the study of inherited diseases, such as cystic fibrosis (CF), through somatic cell nuclear transfer (SCNT) has opened up new opportunities for enhancing our understanding of disease pathology and for identifying new therapies. Thus, the development of species-specific in vitro cell systems that will provide broader insight into organ- and cell-type-specific functions relevant to the pathology of the disease is crucial. Studies have been undertaken to establish transformed rabbit airway epithelial cell lines that display differentiated features characteristic of the primary airway epithelium. This study describes the successful establishment and characterization of two SV40-transformed rabbit tracheal epithelial cell lines. These cell lines, 5RTEo- and 9RTEo-, express the CF transmembrane conductance regulator (CFTR) gene, retain epithelial-specific differentiated morphology and show CFTR-based cAMP-dependent Cl− ion transport across the apical membrane of a confluent monolayer. Immunocytochemical analysis indicates the presence of airway cytokeratins and tight-junction proteins in the 9RTEo- cell line after multiple generations. However, the tight junctions appear to diminish in their efficacy in both cell lines after at least 100 generations. Initial SCNT studies with the 9RTEo- cells have revealed that SV40-transformed rabbit airway epithelial donor cells can be used to generate blastocysts. These cell systems provide valuable models for studying the developmental and metabolic modulation of CFTR gene expression and rabbit airway epithelial cell biology.
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DOI：
10.1152/ajprenal.1996.271.6.f1147
发表时间：
1996-12-01
影响因子：
4.2
作者：
Burns, KD;Regnier, L;Hebert, RL
通讯作者：
Hebert, RL
影响因子：
2.8
作者：
Gomez, M. C.;Pope, C. E.;Dresser, B. L.
通讯作者：
Dresser, B. L.
DOI：
10.1089/153623003772032808
发表时间：
2003-12-01
期刊：
CLONING AND STEM CELLS
影响因子：
--
作者：
Challah-Jacques, M;Chesne, P;Renard, JP
通讯作者：
Renard, JP
影响因子：
3.7
作者：
Fang, Zhen F.;Gai, Hui;Sheng, Hui Z.
通讯作者：
Sheng, Hui Z.
DOI：
10.1016/j.jcf.2004.05.040
发表时间：
2004-08-01
期刊：
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society
影响因子：
--
作者：
Gruenert, D C;Willems, M;Frizzell, R A
通讯作者：
Frizzell, R A