Genome-wide association analyses for fatty acid composition in porcine muscle and abdominal fat tissues.
Genome-wide association analyses for fatty acid composition in porcine muscle and abdominal fat tissues.
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猪肌肉和腹部脂肪组织中脂肪酸组成的全基因组关联分析
DOI：
10.1371/journal.pone.0065554
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ren J
中科院分区：
文献类型：
--
作者：
Yang B;Zhang W;Zhang Z;Fan Y;Xie X;Ai H;Ma J;Xiao S;Huang L;Ren J
脂肪酸组成是猪的一个重要表型性状，它影响猪肉的营养、技术和感官品质。在这里，我们报道了591只白色Duroc×Erhualian F2动物和282头中国苏泰猪肌肉样本中最长肌和腹部脂肪组织脂肪酸组成的全基因组关联研究（GWAS）。在15条猪染色体（SSC）上共鉴定出12种脂肪酸的46个显著性位点，揭示了猪脂肪酸组成的复杂遗传结构。在46个位点中，SSC5、7、14和16上的15个位点达到全基因组显著水平。两个最显著的snp分别是ss131535508 （P = 2.48×10−25）和ss478935891 (P = 3.29×10−13)，分别在腹部脂肪C20∶0和肌肉C18∶0的SSC16位点上达到41.39 Mb和121.31 Mb。GWAS的荟萃分析发现了4个新的基因座，与单一人群相比，其中6个基因座的关联强度增强，表明存在共同的潜在变异。最长肌和腹部脂肪在大多数已确定的位点上显示一致的关联谱，在一些位点上显示不同的关联信号。除SSC4和SSC7上的两个位点影响多种肥胖性状外，所有位点都对脂肪酸组成有特定影响。在SSC14上C18∶0和C16∶1的先导snp邻近区域发现了几个有希望的候选基因，如SSC14上C18∶0和C16∶1的SCD和SSC16上C20∶0的ELOVL7。该发现提供了对猪脂肪酸组成的分子基础的见解，并将有利于最终确定潜在的突变。
Fatty acid composition is an important phenotypic trait in pigs as it affects nutritional, technical and sensory quality of pork. Here, we reported a genome-wide association study (GWAS) for fatty acid composition in the longissimus muscle and abdominal fat tissues of 591 White Duroc×Erhualian F2 animals and in muscle samples of 282 Chinese Sutai pigs. A total of 46 loci surpassing the suggestive significance level were identified on 15 pig chromosomes (SSC) for 12 fatty acids, revealing the complex genetic architecture of fatty acid composition in pigs. Of the 46 loci, 15 on SSC5, 7, 14 and 16 reached the genome-wide significance level. The two most significant SNPs were ss131535508 (P = 2.48×10−25) at 41.39 Mb on SSC16 for C20∶0 in abdominal fat and ss478935891 (P = 3.29×10−13) at 121.31 Mb on SSC14 for muscle C18∶0. A meta-analysis of GWAS identified 4 novel loci and enhanced the association strength at 6 loci compared to those evidenced in a single population, suggesting the presence of common underlying variants. The longissimus muscle and abdominal fat showed consistent association profiles at most of the identified loci and distinct association signals at several loci. All loci have specific effects on fatty acid composition, except for two loci on SSC4 and SSC7 affecting multiple fatness traits. Several promising candidate genes were found in the neighboring regions of the lead SNPs at the genome-wide significant loci, such as SCD for C18∶0 and C16∶1 on SSC14 and ELOVL7 for C20∶0 on SSC16. The findings provide insights into the molecular basis of fatty acid composition in pigs, and would benefit the final identification of the underlying mutations.
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影响因子：
3.7
作者：
Ramos AM;Crooijmans RP;Affara NA;Amaral AJ;Archibald AL;Beever JE;Bendixen C;Churcher C;Clark R;Dehais P;Hansen MS;Hedegaard J;Hu ZL;Kerstens HH;Law AS;Megens HJ;Milan D;Nonneman DJ;Rohrer GA;Rothschild MF;Smith TP;Schnabel RD;Van Tassell CP;Taylor JF;Wiedmann RT;Schook LB;Groenen MA
通讯作者：
Groenen MA
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
4.5
作者：
Ren J;Duan Y;Qiao R;Yao F;Zhang Z;Yang B;Guo Y;Xiao S;Wei R;Ouyang Z;Ding N;Ai H;Huang L
通讯作者：
Huang L
影响因子：
2.4
作者：
Guo, T.;Ren, J.;Huang, L.
通讯作者：
Huang, L.
影响因子：
158.5
作者：
BORKMAN, M;STORLIEN, LH;CAMPBELL, LV
通讯作者：
CAMPBELL, LV