An effective histological staining process to visualize bone interstitial fluid space using confocal microscopy.
An effective histological staining process to visualize bone interstitial fluid space using confocal microscopy.
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DOI：
10.1016/j.bone.2009.01.376
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发表时间：
2009-05
期刊：
影响因子：
4.1
通讯作者：
Fritton, Susannah R.
中科院分区：
文献类型：
--
作者：
Ciani, Cesare;Doty, Stephen B.;Fritton, Susannah R.
骨是一种复合多孔材料，具有两种功能水平的孔隙率：围绕血管的血管孔隙率和围绕骨细胞的腔隙-小管孔隙率。血管孔隙度和腔隙-小管孔隙度直接参与间质液流动，被认为在骨的维持中起重要作用。由于腔隙-小管孔隙度的小尺寸，间质液空间一直难以可视化和量化。我们报告了一种新的染色协议，是可靠的，易于重现，使用异硫氰酸荧光素（FITC）作为共聚焦显微镜可视化的探针。重建的FITC染色横截面能够有效地观察骨显微结构和微孔。这种新的染色方法可用于分析间质液空间，提供皮质骨和松质骨的血管孔和腔隙-小管网络的高分辨率定量。
Bone is a composite porous material with two functional levels of porosity: the vascular porosity that surrounds blood vessels and the lacunar-canalicular porosity that surrounds the osteocytes. Both the vascular porosity and lacunar-canalicular porosity are directly involved in interstitial fluid flow, thought to play an important role in bone’s maintenance. Because of the small dimensions of the lacunar-canalicular porosity, interstitial fluid space has been difficult to visualize and quantify. We report a new staining protocol that is reliable and easily reproducible, using fluorescein isothiocyanate (FITC) as a probe visualized by confocal microscopy. Reconstructed FITC-stained cross sections enable effective visualization of bone microstructure and microporosities. This new staining process can be used to analyze interstitial fluid space, providing high-resolution quantification of the vascular pores and the lacunar-canalicular network of cortical and cancellous bone.
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影响因子：
4.2
作者：
Cooper, DML;Matyas, JR;Hallgrimsson, B
通讯作者：
Hallgrimsson, B
影响因子：
4.8
作者：
KLEINNULEND, J;VANDERPLAS, A;BURGER, EH
通讯作者：
BURGER, EH
DOI：
10.3109/17453679508995514
发表时间：
1995-04-01
期刊：
ACTA ORTHOPAEDICA SCANDINAVICA
影响因子：
--
作者：
KUSUZAKI, K;KAGEYAMA, N;HIRASAWA, Y
通讯作者：
HIRASAWA, Y
影响因子：
2.4
作者：
WEINBAUM, S;COWIN, SC;ZENG, Y
通讯作者：
ZENG, Y
DOI：
10.1002/ar.a.20050
发表时间：
2004-06-01
期刊：
ANATOMICAL RECORD PART A-DISCOVERIES IN MOLECULAR CELLULAR AND EVOLUTIONARY BIOLOGY
影响因子：
--
作者：
You, LD;Weinbaum, S;Schaffler, MB
通讯作者：
Schaffler, MB