Bacterial processing of glucose modulates C. elegans lifespan and healthspan.
Bacterial processing of glucose modulates C. elegans lifespan and healthspan.
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DOI：
10.1038/s41598-021-85046-3
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发表时间：
2021-03-15
影响因子：
4.6
通讯作者：
Tissenbaum HA
中科院分区：
文献类型：
--
作者：
Kingsley SF;Seo Y;Allen C;Ghanta KS;Finkel S;Tissenbaum HA
肠道微生物群在宿主生物体的健康中起着至关重要的作用。在这里，我们定义了大肠杆菌（E。coli）在长期暴露于葡萄糖后使用秀丽隐杆线虫-E.大肠杆菌系统，其中只有细菌与葡萄糖直接接触。我们的数据表明，细菌对葡萄糖的处理导致寿命和健康寿命的减少，包括减少运动，氧化应激抗性和热应激抗性。优雅的长期暴露于葡萄糖，E。大肠杆菌表现出生长缺陷和增加的晚期糖基化终产物。当E.大肠杆菌不能处理额外的葡萄糖，并通过添加抗糖化化合物肌肽。寄主的生理变化。秀丽线虫伴随着解毒基因的失调，包括谷胱甘肽酶、谷胱甘肽-S-转移酶和超氧化物歧化酶。谷胱甘肽-S-转移酶gst-4的缺失缩短了C.并减弱了动物对葡萄糖喂养的细菌饮食的反应。综上所述，我们揭示了膳食中添加的糖可能会改变肠道微生物E。大肠杆菌减少宿主的寿命和健康寿命，并确定解毒基因在慢性高糖饮食期间维持健康的关键作用。
Intestinal microbiota play an essential role in the health of a host organism. Here, we define how commensal Escherichia coli (E. coli) alters its host after long term exposure to glucose using a Caenorhabditis elegans-E. coli system where only the bacteria have direct contact with glucose. Our data reveal that bacterial processing of glucose results in reduced lifespan and healthspan including reduced locomotion, oxidative stress resistance, and heat stress resistance in C. elegans. With chronic exposure to glucose, E. coli exhibits growth defects and increased advanced glycation end products. These negative effects are abrogated when the E. coli is not able to process the additional glucose and by the addition of the anti-glycation compound carnosine. Physiological changes of the host C. elegans are accompanied by dysregulation of detoxifying genes including glyoxalase, glutathione-S-transferase, and superoxide dismutase. Loss of the glutathione-S-transferase, gst-4 shortens C. elegans lifespan and blunts the animal's response to a glucose fed bacterial diet. Taken together, we reveal that added dietary sugar may alter intestinal microbial E. coli to decrease lifespan and healthspan of the host and define a critical role of detoxification genes in maintaining health during a chronic high-sugar diet.
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