High glucose diets shorten lifespan of Caenorhabditis elegans via ectopic apoptosis induction.
High glucose diets shorten lifespan of Caenorhabditis elegans via ectopic apoptosis induction.
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DOI：
10.4162/nrp.2011.5.3.214
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发表时间：
2011-06
影响因子：
2.4
通讯作者：
Choi SS
中科院分区：
文献类型：
--
作者：
Choi SS
基于碳水化合物的饮食由于碳水化合物快速转化为葡萄糖而迅速增加血糖水平。众所周知，高糖饮食会诱发许多生活方式疾病。在这里，我们证明了高糖饮食通过诱导细胞凋亡缩短秀丽隐杆线虫的寿命。不含葡萄糖饮食的对照成年组存活30天，而喂食10 mg/L D-葡萄糖的动物仅存活20天。在1 - 20 mg/L的葡萄糖浓度范围内，葡萄糖饮食导致的寿命缩短呈剂量依赖性。通过触摸刺激动物运动后测量运动反应时间来检查高糖饮食的衰老效应。葡萄糖饮食降低了动物在成年中期的运动能力。高糖饮食还可诱导C.这是一种有效的机制，可以解释寿命缩短和衰老。用SYTO 12染色，在喂食10 mg/L葡萄糖的蠕虫中发现了凋亡细胞尸体。核心凋亡调控基因CED-3和CED-4的突变抑制了高糖饮食诱导的存活率降低，这表明这些调控因子是葡萄糖诱导的凋亡或寿命缩短所必需的。因此，我们得出结论，高糖饮食有可能诱导体内异位凋亡，导致寿命缩短，伴随着运动能力的丧失。
Diets based on carbohydrates increase rapidly the blood glucose level due to the fast conversion of carbohydrates to glucose. High glucose diets have been known to induce many lifestyle diseases. Here, we demonstrated that high glucose diet shortened the lifespan of Caenorhabditis elegans through apoptosis induction. Control adult groups without glucose diet lived for 30 days, whereas animals fed 10 mg/L of D-glucose lived only for 20 days. The reduction of lifespan by glucose diet showed a dose-dependent profile in the concentration range of glucose from 1 to 20 mg/L. Aging effect of high glucose diet was examined by measurement of response time for locomotion after stimulating movement of the animals by touching. Glucose diet decreased the locomotion capacity of the animals during mid-adulthood. High glucose diets also induced ectopic apoptosis in the body of C. elegans, which is a potent mechanism that can explain the shortened lifespan and aging. Apoptotic cell corpses stained with SYTO 12 were found in the worms fed 10 mg/L of glucose. Mutation of core apoptotic regulatory genes, CED-3 and CED-4, inhibited the reduction of viability induced by high glucose diet, which indicates that these regulators were required for glucose-induced apoptosis or lifespan shortening. Thus, we conclude that high glucose diets have potential for inducing ectopic apoptosis in the body, resulting in a shortened lifespan accompanied with loss of locomotion capacity.
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影响因子：
7.1
作者：
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通讯作者：
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影响因子：
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