Identification of host-pathogen-disease relationships using a scalable multiplex serology platform in UK Biobank.
Identification of host-pathogen-disease relationships using a scalable multiplex serology platform in UK Biobank.
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在英国生物银行使用可扩展的多重血清学平台鉴定宿主-病原体-疾病关系。
DOI：
10.1038/s41467-022-29307-3
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发表时间：
2022-04-05
影响因子：
16.6
通讯作者：
Waterboer T
中科院分区：
文献类型：
--
作者：
Mentzer AJ;Brenner N;Allen N;Littlejohns TJ;Chong AY;Cortes A;Almond R;Hill M;Sheard S;McVean G;UKB Infection Advisory Board;Collins R;Hill AVS;Waterboer T
某些传染性病原体是癌症和其他慢性疾病的公认原因。为了了解这种关系背后的病理机制，我们设计了一个多重血清学平台来测量对来自20种感染因子的45种抗原的定量抗体应答，包括人类疱疹，肝炎，多瘤，乳头状瘤和逆转录病毒，以及沙眼衣原体，幽门螺杆菌和弓形虫，然后分析了9695名英国生物样本库参与者的随机子集。我们发现血清阳性率估计值与先前文献的预期一致，并证实抗体应答与社会人口学特征（例如，与C的终身性伴侣。沙眼）、HLA遗传变异（rs6927022与EB病毒（EBV）EBNA 1抗体）和疾病结果（人乳头瘤病毒-16血清阳性与宫颈上皮内瘤变，以及EBV应答与多发性硬化）。我们的可访问数据集是英国前瞻性队列中最大的包含不同传染因子的数据集之一，为我们提供了机会，以提高我们对具有重大临床和公共卫生影响的宿主-病原体-疾病关系的理解。在这里，作者设计了一个多重血清学平台，以定量测量英国生物库参与者对20种传染性病原体的抗体，并确认抗体反应与社会人口学特征，HLA遗传变异和疾病结局的相关性。
Certain infectious agents are recognised causes of cancer and other chronic diseases. To understand the pathological mechanisms underlying such relationships, here we design a Multiplex Serology platform to measure quantitative antibody responses against 45 antigens from 20 infectious agents including human herpes, hepatitis, polyoma, papilloma, and retroviruses, as well as Chlamydia trachomatis, Helicobacter pylori and Toxoplasma gondii, then assayed a random subset of 9695 UK Biobank participants. We find seroprevalence estimates consistent with those expected from prior literature and confirm multiple associations of antibody responses with sociodemographic characteristics (e.g., lifetime sexual partners with C. trachomatis), HLA genetic variants (rs6927022 with Epstein-Barr virus (EBV) EBNA1 antibodies) and disease outcomes (human papillomavirus-16 seropositivity with cervical intraepithelial neoplasia, and EBV responses with multiple sclerosis). Our accessible dataset is one of the largest incorporating diverse infectious agents in a prospective UK cohort offering opportunities to improve our understanding of host-pathogen-disease relationships with significant clinical and public health implications. Here, the authors design a multiplex serology platform to quantitatively measure antibodies against 20 infectious agents in UK Biobank participants and confirm associations of antibody responses with sociodemographic characteristics, HLA genetic variants, and disease outcomes.
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影响因子：
64.8
作者：
Bycroft C;Freeman C;Petkova D;Band G;Elliott LT;Sharp K;Motyer A;Vukcevic D;Delaneau O;O'Connell J;Cortes A;Welsh S;Young A;Effingham M;McVean G;Leslie S;Allen N;Donnelly P;Marchini J
通讯作者：
Marchini J
影响因子：
3.8
作者：
Dondog, Bolormaa;Schnitzler, Paul;Waterboer, Tim
通讯作者：
Waterboer, Tim
影响因子：
51.1
作者：
Cogliano, V;Baan, R;El Ghissassi, F
通讯作者：
El Ghissassi, F
影响因子：
3.7
作者：
Brenner, Nicole;Mentzer, Alexander J.;Waterboer, Tim
通讯作者：
Waterboer, Tim
影响因子：
5.5
作者：
Aebi, C;Fischer, K;Mühlemann, K
通讯作者：
Mühlemann, K