Near-infrared-conjugated humanized anti-carcinoembryonic antigen antibody targets colon cancer in an orthotopic nude-mouse model.
Near-infrared-conjugated humanized anti-carcinoembryonic antigen antibody targets colon cancer in an orthotopic nude-mouse model.
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DOI：
10.1016/j.jss.2017.05.069
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发表时间：
2017-10
期刊：
影响因子：
--
通讯作者：
Bouvet M
中科院分区：
文献类型：
--
作者：
DeLong JC;Murakami T;Yazaki PJ;Hoffman RM;Bouvet M
癌症治愈手术的成功取决于所有癌细胞的完全清除。通过荧光抗体靶向可以增强外科医生的肿瘤可视化。为了进一步开发这种技术，我们选择了与近红外（NIR）染料缀合的人源化抗癌胚抗原（CEA）来靶向裸鼠中原位植入的人类结肠癌。 HT-29人结肠癌细胞系在培养物中生长并皮下注射到小鼠体内。生长3周后，切除肿瘤并切成2mm3的碎片，缝合到另外5只裸鼠的盲肠上进行原位植入。让肿瘤生长4周，此时第3个肿瘤已成功原位生长，并被选择注射与NIR染料IRDye800CW缀合的人源化抗CEA抗体（抗CEA-IRDye800CW）。通过尾静脉注射施用抗体-染料缀合物（75μg）。使用具有 700 nm 和 800 nm 通道的 Pearl Trilogy 小动物成像系统（LI-COR，林肯，内布拉斯加州）获得图像，并使用 Image Studio 进行评估。标记肿瘤后24小时进行剖腹手术。当通过 800 nm 通道成像时，观察到肿瘤被抗 CEA-IRDye800 强烈标记。 48 小时时重复剖腹手术，再次证明通过 800 nm 通道对肿瘤进行了强烈标记，但与 24 小时时成像的 3 只小鼠相比，绝对强度较低（以相对单位表示）。人源化抗CEA-IRDye800CW可以在原位裸鼠模型中快速有效地标记表达CEA的人结肠癌。鉴于该技术能够高度特异性地靶向和标记肿瘤，目前正在开发抗 CEA-IRDye800CW，用于荧光引导手术的临床应用。
The success of a curative surgery for cancer is dependent on the complete removal of all cancer cells. Tumor visualization by the surgeon can be enhanced through fluorescent-antibody targeting. To further develop such technology, we selected humanized anti-carcinoembryonic antigen (CEA) conjugated to a near-infrared (NIR) dye to target orthotopically implanted human colon cancer in nude mice. The HT-29 human colon cancer cell line was grown in culture and subcutaneously injected subcutaneously in mice. After 3 weeks of growth, tumors were resected and cut into 2 mm3 fragments that were sutured to the cecum of 5 additional nude mice for orthotopic implantation. The tumors were allowed to grow for 4 weeks at which point 3 had successful orthotopic tumor growth and were selected for injection of the humanized anti-CEA antibody conjugated to the NIR dye IRDye800CW (anti-CEA-IRDye800CW). The antibody-dye conjugate (75 μg) was administered via tail vein injection. Images were obtained with the Pearl Trilogy Small Animal Imaging System (LI-COR, Lincoln, NE) with both 700 nm and 800 nm channels and evaluated using Image Studio. Laparotomy was performed 24 hours after labeling the tumors. When imaged through the 800 nm channel, the tumors were observed to be strongly labeled with anti-CEA-IRDye800. At 48 hours laparotomy was repeated which again demonstrated strong labeling of the tumors through the 800 nm channel, but with a lower absolute intensity (in relative units), than at 24 hours for each of the 3 mice imaged. Humanized anti-CEA-IRDye800CW can rapidly and effectively label CEA-expressing human colon cancer in an orthotopic nude mouse model. Given the ability of this technology to target and label tumors with great specificity, the anti-CEA-IRDye800CW is currently being developed for clinical use in fluorescence-guided surgery.
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使用荧光抗CEA抗体的肝转移有效荧光引导手术。
影响因子：
2.5
作者：
Hiroshima Y;Lwin TM;Murakami T;Mawy AA;Kuniya T;Chishima T;Endo I;Clary BM;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
DOI：
10.1073/pnas.89.12.5645
发表时间：
1992-06-15
影响因子：
11.1
作者：
FU, XY;GUADAGNI, F;HOFFMAN, RM
通讯作者：
HOFFMAN, RM
影响因子：
3.2
作者：
Kaushal, Sharmeela;McElroy, Michele K.;Luiken, George A.;Talamini, Mark A.;Moossa, A. R.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael
DOI：
10.1007/s00464-014-3895-x
发表时间：
2015-07
影响因子：
3.1
作者：
Boni, Luigi;David, Giulia;Mangano, Alberto;Dionigi, Gianlorenzo;Rausei, Stefano;Spampatti, Sebastiano;Cassinotti, Elisa;Fingerhut, Abe
通讯作者：
Fingerhut, Abe
DOI：
10.1073/pnas.88.20.9345
发表时间：
1991-10-01
影响因子：
11.1
作者：
FU, XY;BESTERMAN, JM;HOFFMAN, RM
通讯作者：
HOFFMAN, RM