Effective fluorescence-guided surgery of liver metastasis using a fluorescent anti-CEA antibody.
Effective fluorescence-guided surgery of liver metastasis using a fluorescent anti-CEA antibody.
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使用荧光抗CEA抗体的肝转移有效荧光引导手术。
DOI：
10.1002/jso.24462
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发表时间：
2016-12
影响因子：
2.5
通讯作者：
Bouvet M
中科院分区：
文献类型：
--
作者：
Hiroshima Y;Lwin TM;Murakami T;Mawy AA;Kuniya T;Chishima T;Endo I;Clary BM;Hoffman RM;Bouvet M
在肿瘤外科手术中，特别是在转移性肿瘤的切除中，充分描绘肿瘤边缘是至关重要的。外科医生在强光手术的可视化方面受到限制，但荧光引导手术（FGS）在帮助外科医生获得负切缘方面是有效的。本研究将FGS用于绿色荧光蛋白（GFP）标记的结直肠肝转移手术原位植入（SOI）小鼠模型。使用与DyLight 650结合的抗cea抗体来突出肿瘤。与GFP相比，荧光抗体在更深的组织深度上清晰地划分病变。anti-CEA-DyLight650在72hr时显示最大的肿瘤-肝脏对比。15只小鼠进行了强光手术（BLS），对照含有GFP的FGS和含有抗cea - dylight650的FGS。接受FGS治疗的小鼠肿瘤残留面积明显减小（P < 0.001），总生存期明显延长（P < 0.001），无病生存期明显延长（P < 0.001）。在两个FGS组中，与仅标记GFP相比，使用抗cea - dylight650进行手术的小鼠存活率提高。在本报告中，我们证明了一种与DyLight 650 nm染料结合的抗cea抗体清楚地标记了结肠癌肝转移，从而实现了成功的FGS。
Delineation of adequate tumor margins is critical in oncologic surgery, particularly in resection of metastatic lesions. Surgeons are limited in visualization with bright-light surgery, but fluorescence-guided surgery (FGS) has been efficacious in helping the surgeon achieve negative margins. The present study uses FGS in a mouse model that has undergone surgical orthotopic implantation (SOI) of colorectal liver metastasis tagged with green fluorescent protein (GFP). An anti-CEA antibody conjugated to DyLight 650 was used to highlight the tumor. The fluorescent antibody clearly demarcated the lesion at deeper tissue depth compared to GFP. Fluorescence of the anti-CEA-DyLight650 showed maximal tumor-to-liver contrast at 72 hr. Fifteen mice underwent bright-light surgery (BLS) versus FGS with GFP versus FGS with anti-CEA-DyLight650. Mice that underwent FGS had a significantly smaller area of residual tumor (P < 0.001) and significantly longer overall survival (P < 0.001) and disease-free survival (P < 0.001). Within the two FGS groups, mice undergoing surgery with anti-CEA-DyLight650 improved survival compared to only GFP labeling. In the present report, we demonstrate that an anti-CEA antibody conjugated to a DyLight 650 nm dye clearly labeled colon cancer liver metastases, thereby enabling successful FGS.
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影响因子：
--
作者：
Tran Cao HS;Kaushal S;Metildi CA;Menen RS;Lee C;Snyder CS;Messer K;Pu M;Luiken GA;Talamini MA;Hoffman RM;Bouvet M
通讯作者：
Bouvet M
DOI：
10.1016/j.jamcollsurg.2012.02.021
发表时间：
2012-07
影响因子：
5.2
作者：
Metildi, Cristina A.;Kaushal, Sharmeela;Hardamon, Chanae R.;Snyder, Cynthia S.;Pu, Minya;Messer, Karen S.;Talamini, Mark A.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael
影响因子：
2.2
作者：
Hiroshima, Yukihiko;Maawy, Ali;Sato, Sho;Murakami, Takashi;Uehara, Fuminari;Miwa, Shinji;Yano, Shuya;Momiyama, Masashi;Chishima, Takashi;Tanaka, Kuniya;Bouvet, Michael;Endo, Itaru;Hoffman, Robert M.
通讯作者：
Hoffman, Robert M.
影响因子：
3.2
作者：
Kaushal, Sharmeela;McElroy, Michele K.;Luiken, George A.;Talamini, Mark A.;Moossa, A. R.;Hoffman, Robert M.;Bouvet, Michael
通讯作者：
Bouvet, Michael
原位裸小鼠模型中肝转移的荧光引导手术。
影响因子：
3.7
作者：
Murakami T;Hiroshima Y;Zhang Y;Chishima T;Tanaka K;Bouvet M;Endo I;Hoffman RM
通讯作者：
Hoffman RM