Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells.
Enhanced on-chip SERS based biomolecular detection using electrokinetically active microwells.
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DOI：
10.1039/b809702j
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发表时间：
2009-02-07
期刊：
影响因子：
6.1
通讯作者：
Erickson D
中科院分区：
文献类型：
--
作者：
Huh YS;Chung AJ;Cordovez B;Erickson D
在这里，我们提出了一种新的微流控技术芯片上的表面增强拉曼光谱（Sers）为基础的生物分子检测，利用电动活性微孔的使用。简而言之，芯片包括一系列包含嵌入式微孔的微流体通道，当电致动时，通过动电效应的组合从溶液中局部吸引或排斥物质。我们证明，该方法结合了现有的同质（溶液相）和异质（表面相）的芯片上技术的优点，使主动混合，以提高Sers增强剂和生物分子目标之间的结合率，以及快速浓度的产品表面相光学询问。本文介绍了芯片的设计和制造过程中，实验结果说明我们的浓度和混合过程的最佳条件，和流动模式的数值分析。为了证明该装置的有用性，我们将其应用于与登革病毒血清型2相关的核酸序列的定量检测。我们报告了30 pM的登革热序列的检测限，并对其他血清型表现出良好的特异性。
Here we present a novel microfluidic technique for on-chip surface enhanced Raman spectroscopy (SERS) based biomolecular detection, exploiting the use of electrokinetically active microwells. Briefly, the chip comprises of a series of microfluidic channels containing embedded microwells that, when electrically actuated, either locally attract or repulse species from solution through a combination of electrokinetic effects. We demonstrate that the approach combines the advantages of existing homogeneous (solution phase) and heterogeneous (surface phase) on-chip techniques by enabling active mixing to enhance the rate of binding between the SERS enhancers and the biomolecular targets as well as rapid concentration of the product for surface phase optical interrogation. This paper describes the chip design and fabrication procedure, experimental results illustrating the optimal conditions for our concentration and mixing processes, and a numerical analysis of the flow pattern. To demonstrate the usefulness of the device we apply it to the quantitative detection of nucleic acid sequences associated with Dengue virus serotype 2. We report a limit of detection for Dengue sequences of 30 pM and show excellent specificity against other serotypes.
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