RFamidergic neurons in the olfactory centers of the terrestrial slug Limax.
RFamidergic neurons in the olfactory centers of the terrestrial slug Limax.
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DOI：
10.1186/s40851-018-0108-9
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发表时间：
2018
影响因子：
2.7
通讯作者：
Matsuo R
中科院分区：
文献类型：
--
作者：
Matsuo Y;Yamanaka A;Matsuo R
陆生蛞蝓Limax长期以来一直被用作研究嗅觉信息加工和气味学习的模型。来自嗅觉上皮的嗅觉输入在触须神经节中加工，然后在前脑中加工。谷氨酸和乙酰胆碱是前脑中使用的主要神经递质。已证实Phe-Met-Arg-Phe-NH2 （FMRFamide）参与调节大脑过程的网络活动。尽管在软体动物的中枢神经系统中，除了FMRFamide外，还有多种RFamide家族肽可能被抗FMRFamide抗体识别，但在Limax中鉴定整个RFamide肽库尚未实现。在本研究中，我们从Limax的转录组数据中全面搜索RFamide肽编码基因，鉴定出12个基因。利用原位杂交技术构建了RFamide基因在包括前脑在内的大脑神经节和上、下触须中的表达图谱。12个基因中有10个基因在前脑表达，9个基因在触手神经节表达。gly - ser - leu - ph - arg - ph - nh2 （GSLFRFamide）由两个不同基因LFRFamide1 （leu - ph - arg - nh2 - 1）和LFRFamide2 （leu - ph - arg - ph - nh2 - 2）编码，外源作用于体外时，降低了前脑局部场电位振荡的振荡频率。免疫组化还发现，表达足部肽的神经元可将前脑的传出投射信号传递回触手神经节。我们的研究结果表明，参与嗅觉信息处理的RFamide家族肽比之前认为的要广泛得多。本文的在线版本（10.1186/s40851-018-0108-9）包含补充材料，可供授权用户使用。
The terrestrial slug Limax has long been used as a model for the study of olfactory information processing and odor learning. Olfactory inputs from the olfactory epithelium are processed in the tentacular ganglion and then in the procerebrum. Glutamate and acetylcholine are the major neurotransmitters used in the procerebrum. Phe-Met-Arg-Phe-NH2 (FMRFamide) has been shown to be involved in the regulation of the network activity of the procerebrum. Although there are thought to be various RFamide family peptides other than FMRFamide that are potentially recognized by anti-FMRFamide antibody in the central nervous system of mollusks, identifying the entire repertoire of RFamide peptides in Limax has yet to be achieved. In the present study, we made a comprehensive search for RFamide peptide-encoding genes from the transcriptome data of Limax, and identified 12 genes. The expression maps of these RFamide genes were constructed by in situ hybridization in the cerebral ganglia including the procerebrum, and in the superior/inferior tentacles. Ten of 12 genes were expressed in the procerebrum, and nine of 12 genes were expressed in the tentacular ganglia. Gly-Ser-Leu-Phe-Arg-Phe-NH2 (GSLFRFamide), which is encoded by two different genes, LFRFamide1 (Leu-Phe-Arg-Phe-NH2–1) and LFRFamide2 (Leu-Phe-Arg-Phe-NH2–2), decreased the oscillatory frequency of the local field potential oscillation in the procerebrum when exogenously applied in vitro. We also found by immunohistochemistry that the neurons expressing pedal peptide send efferent projections from the procerebrum back to the tentacular ganglion. Our findings suggest the involvement of a far wider variety of RFamide family peptides in the olfactory information processing in Limax than previously thought. The online version of this article (10.1186/s40851-018-0108-9) contains supplementary material, which is available to authorized users.
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影响因子：
3.1
作者：
Elekes, Karoly;Battonyai, Izabella;Ito, Etsuro
通讯作者：
Ito, Etsuro
影响因子：
2.5
作者：
GELPERIN, A;RHINES, LD;TANK, DW
通讯作者：
TANK, DW
影响因子：
2.5
作者：
Ito, Iori;Watanabe, Satoshi;Kirino, Yutaka
通讯作者：
Kirino, Yutaka
影响因子：
5.3
作者：
Jing, Jian;Vilim, Ferdinand S.;Weiss, Klaudiusz R.
通讯作者：
Weiss, Klaudiusz R.
DOI：
10.1016/0300-9629(81)90412-6
发表时间：
1981-01-01
影响因子：
2.3
作者：
CHASE, R
通讯作者：
CHASE, R