Molecular Characterization of a Reemergent Brugia malayi Parasite in Sri Lanka, Suggestive of a Novel Strain.
Molecular Characterization of a Reemergent Brugia malayi Parasite in Sri Lanka, Suggestive of a Novel Strain.
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斯里兰卡的重新发射体布鲁吉亚·马来寄生虫的分子表征，暗示了一种新的菌株。
DOI：
10.1155/2021/9926101
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发表时间：
2021
影响因子：
--
通讯作者：
de Silva NR
中科院分区：
文献类型：
--
作者：
Mallawarachchi CH;Chandrasena TGAN;Withanage GP;Premarathna R;Mallawarachchi SMNSM;Gunawardane NY;Dasanayake RS;Gunarathna D;de Silva NR
斯里兰卡于2016年实现了消除淋巴丝虫病的目标；尽管如此，这种疾病仍然是一个潜在的公共卫生问题。本研究旨在通过基于分子的分析，鉴定四十年后在斯里兰卡重新出现的亚周期性Brugia sp.寄生虫。对从人、犬和猫血液样本中分离的布鲁贾丝虫rDNA的内部转录间隔区2 （ITS-2）特异的泛丝虫引物进行聚合酶链反应，得到一条615 bp的条带，确定该物种为马来布鲁贾丝虫。通过与GenBank序列的比较，发现马来亚芽孢杆菌ITS2序列与pahangi的同源性高于具有相似系统发育证据的马来亚芽孢杆菌。然而，与马来白蛉和彭汉吉白蛉生成的Brugia序列的种间kimura -2参数平均两两差异小于3%。在对新ITS2序列节俭性位点的分析中，A36T、A296G、T373A和G482A的替换使得该序列与pahangi和B. malayi都不同，提示可能是B. malayi和B. pahangi的一个新的遗传变异或杂交菌株。蚊子解剖和异种监测发现，斯里兰卡猫、狗和人之间传播的这种新型马来杆菌的媒介是统一分枝杆菌和环状分枝杆菌。
Sri Lanka achieved elimination status for lymphatic filariasis in 2016; still, the disease remains a potential public health issue. The present study is aimed at identifying a subperiodic Brugia sp. parasite which has reemerged in Sri Lanka after four decades via molecular-based analysis. Polymerase chain reaction performed with pan-filarial primers specific for the internal transcribed spacer region-2 (ITS-2) of the rDNA of Brugia filarial parasites isolated from human, canine, and feline blood samples yielded a 615 bp band establishing the species identity as Brugia malayi. Comparison of the ITS2 sequences of the reemerged B. malayi isolates with GenBank sequences revealed a higher sequence homology with B. pahangi than B. malayi with similar phylogenetic evidence. However, the mean interspecies Kimura-2-parameter pairwise divergence between the generated Brugia sequences with B. malayi and B. pahangi was less than 3%. During the analysis of parsimony sites of the new ITS2 sequences, substitutions at A36T, A296G, T373A, and G482A made the sequences different from both B. pahangi and B. malayi suggesting the possibility of a new genetic variant or a hybrid strain of B. malayi and B. pahangi. Mosquito dissections and xenomonitoring identified M. uniformis and M. annulifera as vectors of this novel strain of B. malayi circulating among cats, dogs, and humans in Sri Lanka.
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影响因子：
3.7
作者：
Mallawarachchi CH;Chandrasena NTGA;Wickramasinghe S;Premaratna R;Gunawardane NYIS;Mallawarachchi NSMSM;de Silva NR
通讯作者：
de Silva NR
影响因子：
1.6
作者：
JAYEWARDENE, L G
通讯作者：
JAYEWARDENE, L G
影响因子：
1.2
作者：
Mejia-Madrid, Hugo H.
通讯作者：
Mejia-Madrid, Hugo H.
影响因子：
1.2
作者：
Maafi, ZT;Subbotin, SA;Moens, M
通讯作者：
Moens, M
影响因子：
2.6
作者：
Rishniw, M;Barr, SC;Alpizar, JLD
通讯作者：
Alpizar, JLD