Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in Laodelphax striatellus
Rice black-streaked dwarf virus P10 promotes phosphorylation of GAPDH (glyceraldehyde-3-phosphate dehydrogenase) to induce autophagy in Laodelphax striatellus
复制标题
水稻黑条矮缩病毒P10促进GAPDH（3-磷酸甘油醛脱氢酶）磷酸化诱导灰飞虱自噬
DOI：
10.1080/15548627.2021.1954773
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发表时间：
2021-07
期刊：
影响因子：
13.3
通讯作者：
Wu Jian-xiang
中科院分区：
文献类型：
--
作者：
Wang Qi;Lu Lina;Zeng Ming;Wang Dan;Zhang Tian-ze;Xie Yi;Gao Shi-bo;Fu Shuai;Zhou Xue-ping;Wu Jian-xiang
摘要自噬是一种重要的先天性和适应性免疫反应，通过引导病原微生物降解而清除病原微生物。动物和植物中的病毒感染也已知会诱导自噬。然而，病毒感染如何诱导自噬在很大程度上是未知的。在这里，我们提供的证据表明，水稻黑条矮缩病毒（RBSDV）感染灰飞虱的早期阶段也可以诱导自噬，导致抑制RBSDV的入侵和积累。我们已经确定RBSDV的主要衣壳蛋白（P10）是自噬的诱导剂。RBSDV P10在体外和体内都能与GAPDH（甘油醛-3-磷酸脱氢酶）特异性相互作用。沉默L.纹状体能显著降低RBSDV感染诱导的自噬活性。此外，我们的研究结果还表明，RBSDV感染和RBSDV P10单独可以促进AMP活化蛋白激酶（AMPK）的磷酸化，导致GAPDH磷酸化和GAPDH从细胞质迁移到细胞核中。striatellus或Sf 9昆虫细胞。一旦进入细胞核，磷酸化GAPDH可以激活自噬以抑制病毒感染。总之，这些数据阐明了RBSDV诱导L. striatellus中，并且表明昆虫载体中的自噬途径参与抗RBSDV先天免疫应答。缩略语3-MA：3-甲基腺嘌呤; AMPK：AMP-活化蛋白激酶; ATG：自噬相关; co-IP：免疫共沉淀; DAPI：4 β，6-二脒基-2-苯基吲哚; dpf：饲喂后天数; dsRNA：双链RNA; GAPDH：甘油醛-3-磷酸脱氢酶; GST：谷胱甘肽-S-转移酶; RBSDV：水稻黑条矮缩病毒; TEM：透射电子显微镜。
ABSTRACT Macroautophagy/autophagy is an important innate and adaptive immune response that can clear microbial pathogens through guiding their degradation. Virus infection in animals and plants is also known to induce autophagy. However, how virus infection induces autophagy is largely unknown. Here, we provide evidence that the early phase of rice black-streaked dwarf virus (RBSDV) infection in Laodelphax striatellus can also induce autophagy, leading to suppression of RBSDV invasion and accumulation. We have determined that the main capsid protein of RBSDV (P10) is the inducer of autophagy. RBSDV P10 can specifically interact with GAPDH (glyceraldehyde-3-phosphate dehydrogenase), both in vitro and in vivo. Silencing of GAPDH in L. striatellus could significantly reduce the activity of autophagy induced by RBSDV infection. Furthermore, our results also showed that both RBSDV infection and RBSDV P10 alone can promote phosphorylation of AMP-activated protein kinase (AMPK), resulting in GAPDH phosphorylation and relocation of GAPDH from the cytoplasm into the nucleus in midgut cells of L. striatellus or Sf9 insect cells. Once inside the nucleus, phosphorylated GAPDH can activate autophagy to suppress virus infection. Together, these data illuminate the mechanism by which RBSDV induces autophagy in L. striatellus, and indicate that the autophagy pathway in an insect vector participates in the anti-RBSDV innate immune response. Abbreviations3-MA: 3-methyladenine; AMPK: AMP-activated protein kinase; ATG: autophagy-related; co-IP: co-immunoprecipitation; DAPI: 4ʹ,6-diamidino-2-phenylindole; dpf: days post-feeding; dsRNA: double-stranded RNA; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; GST: glutathione-S-transferase; RBSDV: Rice black-streaked dwarf virus; TEM: transmission electron microscope.
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