Characteristics of salivary telomere length shortening in preterm infants.
Characteristics of salivary telomere length shortening in preterm infants.
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DOI：
10.1371/journal.pone.0280184
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Piyasena, Chinthika
中科院分区：
文献类型：
--
作者：
Schneper, Lisa M.;Drake, Amanda J.;Dunstan, Taylor;Kotenko, Iulia;Notterman, Daniel A.;Piyasena, Chinthika
探讨胎龄、端粒长度与新生儿端粒缩短率之间的关系。从出生时和一岁时的39名足月儿和出生时的32名早产儿的口腔样本中分离基因组DNA，足月校正年龄（受孕后40周）和校正妊娠持续时间的一岁年龄。通过实时定量PCR测量端粒长度。在门诊或研究访视期间以及从医院记录中收集人口统计学和临床数据。社会经济地位估计使用剥夺类别（DEPCAT）的分数来自受试者的邮政编码的卡斯泰尔斯分数。出生时，早产儿的端粒比足月出生的婴儿长。然而，在一岁时，早产儿和足月儿之间的端粒长度没有差异，这意味着早产儿的端粒缩短率高于足月儿。有趣的是，早产儿在受孕后40周的TL显著长于足月儿出生时的TL，这表明受孕后的时间不是影响缩短率的唯一因素。包括性别、胎儿生长受限、母亲年龄、母亲预定体重指数（BMI）、母亲教育水平和DEPCAT评分在内的几个因素在早产儿组和足月儿组之间也存在差异。早产儿在出生时比足月儿具有更长的端粒。在研究的队列中，早产儿组的端粒缩短率高于足月儿组。这一发现与以前使用脐带血的研究一致，表明出生时检测到的早产儿中较长的TL不会持续存在，并表明使用唾液DNA进行婴儿端粒动力学研究是可以接受的。然而，早产儿在受孕后40周的TL比出生时的足月儿长，这表明除了受孕时间之外的生物因素也会影响缩短率。
To examine the association between gestational age, telomere length (TL) and rate of shortening in newborns. Genomic DNA was isolated from buccal samples of 39 term infants at birth and one year and 32 preterm infants at birth, term-adjusted age (40 weeks post-conception) and age one-year corrected for gestational duration. Telomere length was measured by quantitative real-time PCR. Demographic and clinical data were collected during clinic or research visits and from hospital records. Socioeconomic status was estimated using the deprivation category (DEPCAT) scores derived from the Carstairs score of the subject’s postal code. At birth, preterm infants had longer telomeres than infants born at term. However, there was no difference in telomere length between preterm infants and term infants at one year of age, implying that the rate of telomere shortening was greater in pre-term than term infants. Interestingly, TL at age 40 weeks post-conception in preterm infants was significantly longer than term infant TL at birth, suggesting that time since conception is not the only factor that affects rate of shortening. Several factors, including sex, fetal growth restriction, maternal age, maternal booking body mass index (BMI), mother education level and DEPCAT score, also differed between the preterm and term groups. Preterm infants have longer telomeres than term infants at birth. In the studied cohort, the rate of telomere shortening was greater in the premature group compared with the term infants. This finding agrees with previous studies using cord blood, suggesting that the longer TL in premature infants detected at birth do not persist and demonstrating that use of saliva DNA is acceptable for studies of telomere dynamics in infants. However, that the TL at age 40 weeks post-conception in preterm is longer than term infants at birth suggests that biological factors other than time since conception also affect rate of shortening.
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DOI：
10.1097/qad.0000000000001093
发表时间：
2016-06-01
期刊：
AIDS (London, England)
影响因子：
--
作者：
Gianesin K;Noguera-Julian A;Zanchetta M;Del Bianco P;Petrara MR;Freguja R;Rampon O;Fortuny C;Camós M;Mozzo E;Giaquinto C;De Rossi A
通讯作者：
De Rossi A
影响因子：
4.5
作者：
Aubert G;Baerlocher GM;Vulto I;Poon SS;Lansdorp PM
通讯作者：
Lansdorp PM
影响因子：
3.7
作者：
Hadchouel A;Marchand-Martin L;Franco-Montoya ML;Peaudecerf L;Ancel PY;Delacourt C;EPIPAGEADO study group
通讯作者：
EPIPAGEADO study group
影响因子：
2
作者：
Hanssen LM;Schutte NS;Malouff JM;Epel ES
通讯作者：
Epel ES
影响因子：
9.8
作者：
Entringer, Sonja;Epel, Elissa S.;Lin, Jue;Buss, Claudia;Shahbaba, Babak;Blackburn, Elizabeth H.;Simhan, Hyagriv N.;Wadhwa, Pathik D.
通讯作者：
Wadhwa, Pathik D.