Carbon nanotube electric immunoassay for the detection of swine influenza virus H1N1.
Carbon nanotube electric immunoassay for the detection of swine influenza virus H1N1.
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DOI：
10.1016/j.bios.2011.01.029
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发表时间：
2011-04-15
影响因子：
12.6
通讯作者：
Cui T
中科院分区：
文献类型：
--
作者：
Lee D;Chander Y;Goyal SM;Cui T
开发了一种低成本、无标记、超灵敏的电免疫分析法用于检测猪流感病毒（SIV）H1N1。该分析方法基于单壁碳纳米管（SWCNTs）优异的电学性能。抗体 - 病毒复合物会影响底层单壁碳纳米管薄膜的电导，该薄膜是通过简便的逐层自组装构建的。先进行常规免疫分析的基本步骤，最后对免疫芯片进行电学表征。在分析过程中，免疫芯片的电阻在大分子（如多聚 - L - 赖氨酸、抗猪流感病毒抗体和猪流感病毒）表面吸附时往往会增加。猪流感病毒与抗猪流感病毒抗体结合后的电阻变化用裸器件的电阻进行归一化。用非猪流感病毒进行传感器选择性测试，显示归一化电阻变化为12%作为背景。获得了180 TCID50/毫升猪流感病毒的检测限，这表明该分析方法作为即时检测或监测系统具有潜在应用。这种基于碳纳米管的简便免疫分析方法也有可能用作芯片实验室系统的传感平台。
A low-cost, label-free, ultra-sensitive electric immunoassay is developed for the detection of swine influenza virus (SIV) H1N1. The assay is based on the excellent electrical properties of single-walled carbon nanotubes (SWCNTs). Antibody–virus complexes influence the conductance of underlying SWCNT thin film, which has been constructed by facile layer-by-layer self-assembly. The basic steps of conventional immunoassay are performed followed by the electric characterization of immunochips at the last stage. The resistance of immunochips tends to increase upon surface adsorption of macromolecules such as poly-l-lysine, anti-SIV antibodies, and SIVs during the assay. The resistance shift after the binding of SIV with anti-SIV antibody is normalized with the resistances of bare devices. The sensor selectivity tests are performed with non-SIVs, showing the normalized resistance shift of 12% as a background. The detection limit of 180 TCID50/ml of SIV is obtained suggesting a potential application of this assay as point-of-care detection or monitoring system. This facile CNT-based immunoassay also has the potential to be used as a sensing platform for lab-on-a-chip system.
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影响因子：
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