magu is required for germline stem cell self-renewal through BMP signaling in the Drosophila testis.
magu is required for germline stem cell self-renewal through BMP signaling in the Drosophila testis.
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DOI：
10.1016/j.ydbio.2011.06.022
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
DiNardo S
中科院分区：
文献类型：
--
作者：
Zheng Q;Wang Y;Vargas E;DiNardo S
了解干细胞如何维持在其微环境（生态位）中对于它们在再生医学中的应用至关重要。果蝇雄性生殖系干细胞（GSC）的研究已经成为小生境干细胞生物学的一个范例。已知BMP和JAK-STAT通路对于睾丸中GSC的维持是必需的。然而，我们最近的工作有力地表明，BMP信号是导致GSC自我更新的主要途径。在这里，我们表明，magu控制GSC的维护，通过调节BMP途径。我们发现magu在中枢细胞特异性表达，并在睾丸尖端积累。来自magu突变体的睾丸表现出GSC数量减少，但仍保持正常的体干细胞和中枢细胞群。此外，BMP途径活性降低，而JAK-STAT活化在突变体睾丸中保留。最后，由magu突变引起的GSC损失可以通过过度激活种系中的BMP通路来抑制。
Understanding how stem cells are maintained in their microenvironment (the niche) is vital for their application in regenerative medicine. Studies of Drosophila male germline stem cells (GSCs) have served as a paradigm in niche-stem cell biology. It is known that the BMP and JAK-STAT pathways are necessary for the maintenance of GSCs in the testis. However, our recent work strongly suggests that BMP signaling is the primary pathway leading to GSC self-renewal. Here we show that magu controls GSC maintenance by modulating the BMP pathway. We found that magu was specifically expressed from hub cells, and accumulated at the testis tip. Testes from magu mutants exhibited a reduced number of GSCs, yet maintained a normal population of somatic stem cells and hub cells. Additionally, BMP pathway activity was reduced, whereas JAK-STAT activation was retained in mutant testes. Finally, GSC loss caused by the magu mutation could be suppressed by overactivating the BMP pathway in the germline.
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