Molecular diversity and predictability of Vibrio parahaemolyticus along the Georgian coastal zone of the Black Sea.
Molecular diversity and predictability of Vibrio parahaemolyticus along the Georgian coastal zone of the Black Sea.
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DOI：
10.3389/fmicb.2014.00045
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发表时间：
2014
影响因子：
5.2
通讯作者：
Huq A
中科院分区：
文献类型：
--
作者：
Haley BJ;Kokashvili T;Tskshvediani A;Janelidze N;Mitaishvili N;Grim CJ;Constantin de Magny G;Chen AJ;Taviani E;Eliashvili T;Tediashvili M;Whitehouse CA;Colwell RR;Huq A
副溶血性弧菌是导致海产品相关性胃肠炎的主要原因，也是世界范围内海洋和河口环境的土著成员。从沿着格鲁吉亚黑海海岸采集的水样和浮游生物样品中分离出170株副溶血性弧菌，采集时间为28个月。检测所有分离菌株是否存在tlh、trh和tdh。对一部分菌株进行血清分型，并检测流行性的其他因素和标志物。确定了26种血清型，其中5种与临床相关。尽管所有170株分离株均为tdh、trh和神奈川现象阴性，但7株具有副溶血性弧菌大流行株的GS-PCR序列，27株具有850 bp序列。在黑海的副溶血性弧菌人口估计是基因组异质性的rep-PCR和观察到的血清多样性与rep-PCR基因组多样性没有相关性。使用统计建模来预测作为水温函数的副溶血性弧菌的存在，其中在绿色角地点样品中观察到最强的一致性（总方差百分比= 70，P < 0.001）。结果表明，在黑海的副溶血性弧菌，其中一些携带大流行的标志物，随着水温升高相关的丰富的副溶血性弧菌的增加的多样化的人口。
Vibrio parahaemolyticus is a leading cause of seafood-related gastroenteritis and is also an autochthonous member of marine and estuarine environments worldwide. One-hundred seventy strains of V. parahaemolyticus were isolated from water and plankton samples collected along the Georgian coast of the Black Sea during 28 months of sample collection. All isolated strains were tested for presence of tlh, trh, and tdh. A subset of strains were serotyped and tested for additional factors and markers of pandemicity. Twenty-six serotypes, five of which are clinically relevant, were identified. Although all 170 isolates were negative for tdh, trh, and the Kanagawa Phenomenon, 7 possessed the GS-PCR sequence and 27 the 850 bp sequence of V. parahaemolyticus pandemic strains. The V. parahaemolyticus population in the Black Sea was estimated to be genomically heterogeneous by rep-PCR and the serodiversity observed did not correlate with rep-PCR genomic diversity. Statistical modeling was used to predict presence of V. parahaemolyticus as a function of water temperature, with strongest concordance observed for Green Cape site samples (Percent of total variance = 70, P < 0.001). Results demonstrate a diverse population of V. parahaemolyticus in the Black Sea, some of which carry pandemic markers, with increased water temperature correlated to an increase in abundance of V. parahaemolyticus.
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影响因子：
2.2
作者：
Bej, AK;Patterson, DP;Kaysner, CA
通讯作者：
Kaysner, CA
影响因子：
2.8
作者：
Chao, Guoxiang;Jiao, Xinan;Qian, Xiaoqin
通讯作者：
Qian, Xiaoqin
影响因子：
4.2
作者：
Clark, CG;Kravetz, AN;Johnson, WM
通讯作者：
Johnson, WM
DOI：
10.1073/pnas.1207359109
发表时间：
2012-07-17
影响因子：
11.1
作者：
Hasan, Nur A.;Choi, Seon Young;Colwell, Rita R.
通讯作者：
Colwell, Rita R.
影响因子：
3.4
作者：
Kodama, Toshio;Rokuda, Mitsuhiro;Honda, Takeshi
通讯作者：
Honda, Takeshi