Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells.
Co-expression of two subtypes of melatonin receptor on rat M1-type intrinsically photosensitive retinal ganglion cells.
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DOI：
10.1371/journal.pone.0117967
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Weng SJ
中科院分区：
文献类型：
--
作者：
Sheng WL;Chen WY;Yang XL;Zhong YM;Weng SJ
视网膜内光敏视网膜神经节细胞（ipRGC）参与昼夜节律和其他非图像形成视觉响应。一个悬而未决的问题是，这些神经元的活动是否也可能在神经激素褪黑激素介导的调节下。本研究采用免疫组织化学双染技术，研究了哺乳动物褪黑素受体的两种亚型MT1和MT2在大鼠ipRGCs中的表达。由针对黑视素的特异性抗体标记的视网膜神经节细胞的单个子集表现出典型的M1型ipRGC的形态。MT1和MT2受体的免疫反应性在所有标记的ipRGC的细胞质中清楚地观察到，表明这两种受体在这些神经元中的每一个中共表达。此外，早在出生当天就在新生儿M1细胞中发现了这两种受体的标记。因此，视网膜褪黑激素可以直接调节ipRGC的活性，从而调节非成像视觉功能是非常合理的。
Intrinsically photosensitive retinal ganglion cells (ipRGCs) are involved in circadian and other non-image forming visual responses. An open question is whether the activity of these neurons may also be under the regulation mediated by the neurohormone melatonin. In the present work, by double-staining immunohistochemical technique, we studied the expression of MT1 and MT2, two known subtypes of mammalian melatonin receptors, in rat ipRGCs. A single subset of retinal ganglion cells labeled by the specific antibody against melanopsin exhibited the morphology typical of M1-type ipRGCs. Immunoreactivity for both MT1 and MT2 receptors was clearly seen in the cytoplasm of all labeled ipRGCs, indicating that these two receptors were co-expressed in each of these neurons. Furthermore, labeling for both the receptors were found in neonatal M1 cells as early as the day of birth. It is therefore highly plausible that retinal melatonin may directly modulate the activity of ipRGCs, thus regulating non-image forming visual functions.
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