Comparison of the behavior of neural stem cells in the brain of normal and twitcher mice after neonatal transplantation.
Comparison of the behavior of neural stem cells in the brain of normal and twitcher mice after neonatal transplantation.
复制标题
新生移植后正常小鼠和抽搐小鼠大脑中神经干细胞行为的比较。
DOI：
10.1089/scd.2006.0092
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发表时间：
2007
影响因子：
4
通讯作者：
R. Taylor
中科院分区：
文献类型：
--
作者：
Guoying Zhao;N. McCarthy;P. Sheehy;R. Taylor
抽动鼠是人类Krabbe病的模型，由半乳糖脑苷酶基因突变引起。由于髓鞘分解代谢不足，少突胶质细胞死亡和脱髓鞘广泛存在于中枢和外周神经系统，是研究髓鞘修复策略的理想模型。在这里，我们描述了表达增强型绿色荧光蛋白(EGFP)的小鼠神经干细胞(NSCs)在新生正常和抽动小鼠中的移植应用。各年龄组(20、30、45d龄)的正常和Twitcher小鼠均表现出移植细胞的植入和分化。移植的细胞在脑室和脑实质的广泛区域中被发现。30天龄正常小鼠和抽动小鼠在植入细胞总数和植入模式方面没有显著差异。30d龄小鼠脑内平均移植细胞数为964+/-281(n=8)。移植的细胞具有神经元、星形胶质细胞和少突胶质细胞的形态。通过使用细胞类型特异性标记物的免疫组织化学染色证实分化为少突胶质细胞。在正常和抽动小鼠的大脑中，移植到灰质的细胞比例都高于白质(p<；0.01)。本研究表明，30天龄Twitcher鼠脑内脱髓鞘的环境并未明显改变新生移植后NSCs的植入和分布模式。
The twitcher mouse is a model of human Krabbe's disease caused by a mutation in the galacto-cerebrosidase gene. As a result of deficient catabolism of myelin, death of oligodendrocytes and demyelination occur widely in the central and peripheral nervous system, making it an ideal model for investigation of myelin repair strategies. Here we describe the use of mouse neural stem cells (NSCs) expressing enhanced green fluorescence protein (eGFP) for transplantation in neonatal normal and twitcher mice. Normal and twitcher mice in all age groups (20, 30, and 45 days old) showed engraftment and differentiation of injected cells. The engrafted cells were found in the ventricles and a wide range of regions in the brain parenchyma. There was no significant difference in the total number of cells engrafted and the pattern of engraftment between 30-day-old normal and twitcher mice. The average number of engrafted cells in the brain of a 30-day-old mouse was 964 +/- 281 (n = 8). Engrafted cells with the morphology of neurons, astrocytes, and oligodendrocytes were identified. Differentiation into oligodendrocytes was confirmed by immunohistochemical staining using a cell-type-specific marker. There was a higher percentage of cells engrafted in the grey matter than in the white matter (p < 0.01) in both normal and twitcher mouse brain. This study indicates that the environment of demyelination in 30-day-old twitcher mouse brain has not significantly altered the engraftment and distribution patterns of NSCs after neonatal transplantation.
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DOI：
10.1073/pnas.97.11.6126
发表时间：
2000-05-23
影响因子：
11.1
作者：
Liu, S;Qu, Y;McDonald, JW
通讯作者：
McDonald, JW
影响因子：
56.9
作者：
Brüstle, O;Jones, KN;McKay, RDG
通讯作者：
McKay, RDG
影响因子：
0.9
作者：
Rosanne M. Taylor;Evan Y. Snyder
通讯作者：
Rosanne M. Taylor;Evan Y. Snyder
影响因子：
158.5
作者：
Escolar, ML;Poe, MD;Kurtzberg, J
通讯作者：
Kurtzberg, J
DOI：
10.1002/neu.480210209
发表时间：
1990-03-01
期刊：
JOURNAL OF NEUROBIOLOGY
影响因子：
--
作者：
RYDER, EF;SNYDER, EY;CEPKO, CL
通讯作者：
CEPKO, CL