Epithelium-dependent extracellular matrix synthesis in transforming growth factor-beta 1-growth-inhibited mouse mammary gland.
Epithelium-dependent extracellular matrix synthesis in transforming growth factor-beta 1-growth-inhibited mouse mammary gland.
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DOI：
10.1083/jcb.110.6.2209
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发表时间：
1990-06
期刊：
影响因子：
--
通讯作者：
Daniel CW
中科院分区：
文献类型：
--
作者：
Silberstein GB;Strickland P;Coleman S;Daniel CW
早期研究表明，外源性转化生长因子β(TGF-β1)对小鼠乳腺导管生长有可逆性抑制作用。使用小型塑料植入物原位治疗乳腺发育区域，我们现在报告，转化生长因子-β1生长抑制与I型胶原信使RNA和蛋白质以及糖胺多糖和硫酸软骨素的异位积累有关。这两个大分子都是导管细胞外基质的正常成分，在外源性转化生长因子-β1的影响下，它们异常地集中在导管生长点顶端的上皮细胞附近，即末端芽。细胞外基质的刺激仅限于受影响的末端芽周围结缔组织细胞的聚集，而不存在于转化生长因子-β1植入物本身，表明基质效应是上皮依赖的。异位基质合成是转化生长因子-β1所特有的，因为它在用其他生长抑制剂处理的导管中不存在，或者在经历正常内源性调节过程的导管中不存在。这些发现与其他系统中报道的转化生长因子-β1的基质刺激特性是一致的，但不同之处在于它们对上皮的严格依赖。内源性转化生长因子-β1可能在调节乳腺上皮-间质相互作用中起作用。
Exogenous transforming growth factor beta (TGF-beta 1) was shown in earlier studies to reversibly inhibit mouse mammary ductal growth. Using small plastic implants to treat regions of developing mammary glands in situ, we now report that TGF-beta 1 growth inhibition is associated with an ectopic accumulation of type I collagen messenger RNA and protein, as well as the glycosaminoglycan, chondroitin sulfate. Both macromolecules are normal components of the ductal extracellular matrix, which, under the influence of exogenous TGF-beta 1, became unusually concentrated immediately adjacent to the epithelial cells at the tip of the ductal growth points, the end buds. Stimulation of extracellular matrix was confined to aggregations of connective tissue cells around affected end buds and was not present around the TGF-beta 1 implants themselves, indicating that the matrix effect was epithelium dependent. Ectopic matrix synthesis was specific for TGF-beta 1 insofar as it was absent at ducts treated with other growth inhibitors, or at ducts undergoing normal involution in response to endogenous regulatory processes. These findings are consistent with the matrix-stimulating properties of TGF-beta 1 reported for other systems, but differ in their strict dependence upon epithelium. A possible role for endogenous TGF-beta 1 in modulating a mammary epithelium-stroma interaction is suggested.
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影响因子：
64.5
作者：
KIMELMAN, D;KIRSCHNER, M
通讯作者：
KIRSCHNER, M
影响因子：
3.2
作者：
LOPEZDELEON, A;ROJKIND, M
通讯作者：
ROJKIND, M
影响因子：
2.7
作者：
COLEMAN, S;DANIEL, CW
通讯作者：
DANIEL, CW
影响因子：
6.5
作者：
FALANGA, V;TIEGS, SL;SPORN, MB
通讯作者：
SPORN, MB
DOI：
10.1073/pnas.81.15.4950
发表时间：
1984-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SILBERSTEIN, GB;STRICKLAND, P;DANIEL, CW
通讯作者：
DANIEL, CW