Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation.
Bone marrow-derived cells rescue salivary gland function in mice with head and neck irradiation.
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DOI：
10.1016/j.biocel.2010.09.023
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发表时间：
2011-01
影响因子：
4
通讯作者：
Tran, Simon D.
中科院分区：
文献类型：
--
作者：
Sumita, Yoshinori;Liu, Younan;Khalili, Saeed;Maria, Ola M.;Xia, Dengsheng;Key, Sharon;Cotrim, Ana P.;Mezey, Eva;Tran, Simon D.
大多数头颈部癌症患者的治疗包括电离辐射。这种治疗的后果是唾液腺（SGs）的不可逆损伤，这伴随着分泌液体的腺泡细胞的丧失和唾液分泌的大量减少。虽然目前没有足够的常规治疗方法，但基于细胞的治疗方法越来越受到关注。在这项研究中，我们研究了骨髓源性细胞（bmdc）是否能够分化为唾液上皮细胞并恢复头颈部辐照小鼠的SG功能。将雄性小鼠的BMDCs移植到18只gy照射的雌性小鼠的尾静脉中。在照射后第8周和第24周接受BMDCs移植的小鼠唾液分泌量增加。照射（IR）后24周，bmdc治疗小鼠收获的SGs（下颌骨和腮腺）比未治疗小鼠的体重更大。组织学分析表明，SGs处理小鼠的组织再生活性（如血管形成和细胞增殖）水平增加，而未移植小鼠的凋亡活性增加。在bmdc处理的SGs中检测到干细胞标记物（Sca-1或c-kit）的表达。最后，我们检测到bmdc处理小鼠的腺泡细胞面积比例增加，y染色体阳性（供体来源）唾液上皮细胞约占9%。我们在此提出，使用BMDCs进行细胞治疗可以通过将供体BMDCs直接分化为唾液腺上皮细胞来挽救受辐射SGs的功能损伤。
Treatment for most patients with head and neck cancers includes ionizing radiation. A consequence of this treatment is irreversible damage to salivary glands (SGs), which is accompanied by a loss of fluid-secreting acinar-cells and a considerable decrease of saliva output. While there are currently no adequate conventional treatments for this condition, cell-based therapies are receiving increasing attention to regenerate SGs. In this study, we investigated whether bone marrow-derived cells (BMDCs) can differentiate into salivary epithelial cells and restore SG function in head and neck irradiated mice. BMDCs from male mice were transplanted into the tail-vein of 18 Gy-irradiated female mice. Salivary output was increased in mice that received BMDCs transplantation at week 8 and 24 post-irradiation. At 24 weeks after irradiation (IR), harvested SGs (submandibular and parotid glands) of BMDC-treated mice had greater weights than those of non-treated mice. Histological analysis shows that SGs of treated mice demonstrated an increased level of tissue regenerative activity such as blood vessel formation and cell proliferation, while apoptotic activity was increased in non-transplanted mice. The expression of stem cell markers (Sca-1 or c-kit) was detected in BMDC-treated SGs. Finally, we detected an increased ratio of acinar-cell area and approximately 9% of Y-chromosome-positive (donor-derived) salivary epithelial cells in BMDC-treated mice. We propose here that cell therapy using BMDCs can rescue the functional damage of irradiated SGs by direct differentiation of donor BMDCs into salivary epithelial cells.
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干细胞移植在辐照腺体中的干细胞移植后拯救唾液腺功能。
影响因子：
3.7
作者：
Lombaert, Isabelle M. A.;Brunsting, Jeanette F.;Wierenga, Pieter K.;Faber, Hette;Stokman, Monique A.;Kok, Tineke;Visser, Willy H.;Kampinga, Harm H.;de Haan, Gerald;Coppes, Robert P.
通讯作者：
Coppes, Robert P.
影响因子：
18.6
作者：
Baum, BJ;Tran, SD
通讯作者：
Tran, SD
影响因子：
5.4
作者：
Tatsuishi, Yuki;Hirota, Makoto;Tohnai, Iwai
通讯作者：
Tohnai, Iwai
影响因子：
--
作者：
Aframian, Doron J.;Amit, Doron;Palmon, Aaron
通讯作者：
Palmon, Aaron
影响因子：
3.3
作者：
Sugito, T;Kagami, H;Ueda, M
通讯作者：
Ueda, M