Fluorescence resonance energy transfer in polydiacetylene liposomes.
Fluorescence resonance energy transfer in polydiacetylene liposomes.
复制标题
聚二乙炔脂质体中的荧光共振能量转移。
DOI：
10.1021/jp804640p
复制
发表时间：
2008-10-23
期刊：
影响因子：
--
通讯作者：
Kohli P
中科院分区：
文献类型：
--
作者：
Li X;Matthews S;Kohli P
共轭聚二乙炔（PDA）具有刺激响应特性，已被广泛研究，以开发高效的传感器。我们在这里报告荧光共振能量转移（FRET）在脂质体合成使用不同摩尔比的丹磺酰标记的二乙炔和二乙炔羧酸单体。二乙炔的光聚合导致交联的PDA脂质体。我们使用稳态电子吸收，发射和荧光各向异性（FA）分析，以表征丹磺酰荧光团（供体）和PDA（受体）之间的热诱导FRET。我们发现，受体与供体的单体比例（拉德）和连接体（连接丹磺酰荧光团的功能部分，在单体中的二乙炔基团）的长度强烈影响FRET。当Rad = 10000时，脂质体溶液在298 ~ 338 K加热时，受体发射强度放大18倍以上。拉德的减少导致受体发射放大减少。这主要归因于供体和受体之间较低的FRET效率和较高的背景信号。我们还发现，当丹磺酰基通过较长且柔性的连接体连接到丁二炔时，加热溶液后PDA发射的FRET扩增比通过较短的连接体高得多。我们将此归因于在脂质体的双层中插入丹磺酰，这导致丹磺酰量子产率增加以及多个受体与有限可用供体的更高相互作用。对于PDA扩增小得多的更短和更刚性的接头，情况并非如此。本研究旨在提高我们对荧光团和基于PDA的缀合脂质体之间的FRET的理解。此外，标记在PDA脂质体上的受体可以与蛋白质、酶和细胞上存在的配体相互作用，这将产生发射传感信号。因此，使用本方法，存在设计基于FRET的高灵敏度和选择性化学和生物化学传感器的机会。
Conjugated polydiacetylene (PDA) possessing stimuli-responsive properties has been intensively investigated for developing efficient sensors. We report here fluorescence resonance energy transfer (FRET) in liposomes synthesized using different molar ratios of dansyl-tagged diacetylene and diacetylene–carboxylic acid monomers. Photopolymerization of diacetylene resulted in cross-linked PDA liposomes. We used steady-state electronic absorption, emission, and fluorescence anisotropy (FA) analysis to characterize the thermal-induced FRET between dansyl fluorophores (donor) and PDA (acceptor). We found that the monomer ratio of acceptor to donor (Rad) and length of linkers (functional part that connects dansyl fluorophores to the diacetylene group in the monomer) strongly affected FRET. For Rad = 10 000, the acceptor emission intensity was amplified by more than 18 times when the liposome solution was heated from 298 to 338 K. A decrease in Rad resulted in diminished acceptor emission amplification. This was primarily attributed to lower FRET efficiency between donors and acceptors and a higher background signal. We also found that the FRET amplification of PDA emissions after heating the solution was much higher when dansyl was linked to diacetylene through longer and flexible linkers than through shorter linkers. We attributed this to insertion of dansyl in the bilayer of the liposomes, which led to an increased dansyl quantum yield and a higher interaction of multiple acceptors with limited available donors. This was not the case for shorter and more rigid linkers where PDA amplification was much smaller. The present studies aim at enhancing our understanding of FRET between fluorophores and PDA-based conjugated liposomes. Furthermore, receptor tagged onto PDA liposomes can interact with ligands present on proteins, enzymes, and cells, which will produce emission sensing signal. Therefore, using the present approach, there exist opportunities for designing FRET-based highly sensitive and selective chemical and biochemical sensors.
登录
查看更多内容
影响因子：
29.4
作者：
Aberem, Maite Bera;Najari, Ahmed;Leclerc, Mario
通讯作者：
Leclerc, Mario
影响因子：
2.9
作者：
BRAMHALL, J
通讯作者：
BRAMHALL, J
DOI：
10.1073/pnas.162375999
发表时间：
2002-08-20
影响因子：
11.1
作者：
Gaylord, BS;Heeger, AJ;Bazan, GC
通讯作者：
Bazan, GC
影响因子：
15
作者：
He, F;Tang, YL;Zhu, DB
通讯作者：
Zhu, DB
影响因子：
15
作者：
Ho, HA;Doré, K;Leclerc, M
通讯作者：
Leclerc, M