The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus).
The Early Peritoneal Cavity Immune Response to Vibrio Anguillarum Infection and to Inactivated Bacterium in Olive Flounder (Paralichthys olivaceus).
复制标题
牙鲆（Paralichthys olivaceus）对鳗弧菌感染和灭活细菌的早期腹腔免疫反应
DOI：
10.3390/microorganisms10112175
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发表时间：
2022-11-02
期刊：
影响因子：
4.5
通讯作者：
中科院分区：
文献类型：
--
作者：
腹腔在免疫应答中起着重要作用，腹腔接种是鱼类理想的疫苗接种途径。然而，硬骨鱼腹腔的免疫反应仍然没有完全的特点。本研究对牙鲆腹腔细胞（PerC细胞）的形态及其组成进行了研究。常驻PerC细胞的流式细胞术分析揭示了粒度和大小不同的两个群体。一个群体，约15.43% ± 1.8%，较小，粒度较低，称为淋巴细胞。另一种细胞群体（78.17% ± 3.52%）体积较大，颗粒度较高，为髓系细胞。细胞化学染色和透射电镜观察结果表明，比目鱼腹腔内通常含有以粒细胞、巨噬细胞、树突状细胞和淋巴细胞为主的白细胞定居群体。流式细胞仪检测PerC细胞中IgM+、CD 4+、G-CSFR+、MHCII+和CD 83+白细胞的百分比分别为3.13% ± 0.4%、2.83% ± 0.53%、21.12% ± 1.44%、27.11% ± 3.30%和19.64% ± 0.31%。同时比较了鳗弧菌感染和免疫后比目鱼IgM+、CD 4+、G-CSFR+、MHCII+和CD 83+白细胞的变化。在感染或疫苗接种处理后，组成迅速变化，包括两个阶段，以吞噬细胞为主的非特异性阶段和以淋巴细胞为主的特异性免疫阶段。由于细菌的毒力效应，感染组表现出比免疫组更强烈和复杂的PerC细胞免疫应答。继我们以前的研究，这是第一个报告的形态和组成的PerC细胞和早期激活的perC细胞在比目鱼响应鳗弧菌感染和疫苗接种。
The peritoneal cavity plays an important role in the immune response, and intraperitoneal administration is an ideal vaccination route in fish. However, immune responses in the peritoneal cavity of teleost fish are still not completely characterized. This study characterized the morphology of peritoneal cavity cells (PerC cells) and their composition in flounder (Paralichthys olivaceus). Flow cytometric analysis of the resident PerC cells revealed two populations varying in granularity and size. One population, approximately 15.43% ± 1.8%, was smaller with a lower granularity, designated as lymphocytes. The other population of the cells, about 78.17% ± 3.52%, was larger with higher granularity and was designated as myeloid cells. The results of cytochemical staining and transmission electron microscopy indicated that peritoneal cavity in flounder normally contains a resident population of leukocytes dominated by granulocytes, macrophages, dendritic cells, and lymphocytes. The percentages of IgM+, CD4+, G-CSFR+, MHCII+, and CD83+ leukocytes among PerC cells determined by flow cytometry were 3.13% ± 0.4%, 2.83% ± 0.53%, 21.12% ± 1.44%, 27.11% ± 3.30%, and 19.64% ± 0.31%, respectively. Further, the changes in IgM+, CD4+, G-CSFR+, MHCII+, and CD83+ leukocytes in flounder after Vibrio anguillarum infection and immunization were compared. The composition changed rapidly after the infection or vaccination treatment and included two stages, a non-specific stage dominated by phagocytes and a specific immune stage dominated by lymphocytes. Due to the virulence effectors of bacteria, the infected group exhibited a more intense and complicated PerC cells immune response than that of the immunization group. Following our previous study, this is the first report on the morphology and composition of PerC cells and the early activation of PerC cells in flounder response to V. anguillarum infection and vaccination.
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影响因子：
4.7
作者：
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通讯作者：
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影响因子：
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Gravningen, Kjersti
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影响因子：
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通讯作者：
Breckpot, Karine
影响因子：
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作者：
Chaves-Pozo, E;Muñoz, P;Meseguer, J
通讯作者：
Meseguer, J