In vivo biofunctional evaluation of hydrogels for disc regeneration.
In vivo biofunctional evaluation of hydrogels for disc regeneration.
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DOI：
10.1007/s00586-013-2998-8
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发表时间：
2014-01
影响因子：
2.8
通讯作者：
Wilke, Hans-Joachim
中科院分区：
文献类型：
--
作者：
Reitmaier, Sandra;Kreja, Ludwika;Gruchenberg, Katharina;Kanter, Britta;Silva-Correia, Joana;Oliveira, Joaquim Miguel;Reis, Rui Luis;Perugini, Valeria;Santin, Matteo;Ignatius, Anita;Wilke, Hans-Joachim
再生策略旨在恢复椎间盘的原始生物功能。有多种不同的生物材料可供使用，它们可能有助于椎间盘再生。在本研究中，在绵羊髓核摘除模型中分别研究了两种用抗血管生成肽功能化并接种骨髓来源单核细胞（BMC）的水凝胶成功的可能性。
在一步操作中，采集髂嵴抽吸物，随后将分离的BMC接种到水凝胶上并植入绵羊椎间盘。对于细胞接种方法，使用了一种基于透明质酸的水凝胶。在离子交联的甲基丙烯酰化结冷胶水凝胶上研究了新开发的血管内皮生长因子（VEGF）阻断剂的抗血管生成潜力。未处理的椎间盘作为髓核摘除对照。使用了24只成年美利奴绵羊。在6周后进行组织学分析，在12周后进行组织学和生物力学分析。
生物力学测试显示，任何植入的椎间盘和髓核摘除的椎间盘之间没有差异。与完整的椎间盘相比，所有植入的椎间盘都明显退变。相反，植入的椎间盘和髓核摘除的椎间盘之间没有显著差异。从6周 - 12周，除了细胞接种的水凝胶外，退变评分和椎间盘高度指数有下降趋势（尽管不显著）。细胞接种的水凝胶略微减缓了退变。
没有一种水凝胶配置能够恢复椎间盘的生物功能。这可能大概是由水凝胶挤出造成的。应高度重视纤维环密封剂的开发，它能有效利用（细胞接种的）水凝胶在椎间盘生物再生和恢复椎间盘功能方面的潜力。
Regenerative strategies aim to restore the original biofunctionality of the intervertebral disc. Different biomaterials are available, which might support disc regeneration. In the present study, the prospects of success of two hydrogels functionalized with anti-angiogenic peptides and seeded with bone marrow derived mononuclear cells (BMC), respectively, were investigated in an ovine nucleotomy model. In a one-step procedure iliac crest aspirates were harvested and, subsequently, separated BMC were seeded on hydrogels and implanted into the ovine disc. For the cell-seeded approach a hyaluronic acid-based hydrogel was used. The anti-angiogenic potential of newly developed VEGF-blockers was investigated on ionically crosslinked metacrylated gellan gum hydrogels. Untreated discs served as nucleotomy controls. 24 adult merino sheep were used. After 6 weeks histological, after 12 weeks histological and biomechanical analyses were conducted. Biomechanical tests revealed no differences between any of the implanted and nucleotomized discs. All implanted discs significantly degenerated compared to intact discs. In contrast, there was no marked difference between implanted and nucleotomized discs. In tendency, albeit not significant, degeneration score and disc height index deteriorated for all but not for the cell-seeded hydrogels from 6 to 12 weeks. Cell-seeded hydrogels slightly decelerated degeneration. None of the hydrogel configurations was able to regenerate biofunctionality of the intervertebral disc. This might presumably be caused by hydrogel extrusion. Great importance should be given to the development of annulus sealants, which effectively exploit the potential of (cell-seeded) hydrogels for biological disc regeneration and restoration of intervertebral disc functioning.
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影响因子：
2.8
作者：
Kandel, Rita;Roberts, Sally;Urban, Jill P. G.
通讯作者：
Urban, Jill P. G.
影响因子：
2.8
作者：
Fernando, Hanan N.;Czamanski, Jessica;Yuan, Tai-Yi;Gu, Weiyong;Salahadin, Abdi;Huang, Chun-Yuh Charles
通讯作者：
Huang, Chun-Yuh Charles
DOI：
10.1111/j.1749-6632.2012.06667.x
发表时间：
2012-01-01
期刊：
HEMATOPOIETIC STEM CELLS VIII
影响因子：
--
作者：
Bernardo, Maria Ester;Fibbe, Willem E.
通讯作者：
Fibbe, Willem E.
影响因子：
2.4
作者：
Hunter, CJ;Matyas, JR;Duncan, NA
通讯作者：
Duncan, NA
影响因子：
3
作者：
Buser, Zorica;Kuelling, Fabrice;Lotz, Jeffrey C.
通讯作者：
Lotz, Jeffrey C.