Over-expression of a cyanobacterial gene for 1-deoxy-d-xylulose-5-phosphate synthase in the chloroplast of Chlamydomonas reinhardtii perturbs chlorophyll: carotenoid ratios.
Over-expression of a cyanobacterial gene for 1-deoxy-d-xylulose-5-phosphate synthase in the chloroplast of Chlamydomonas reinhardtii perturbs chlorophyll: carotenoid ratios.
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DOI：
10.1016/j.jksus.2022.102141
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发表时间：
2022-08
影响因子：
3.8
通讯作者：
Purton, Saul
中科院分区：
文献类型：
--
作者：
Al Hoqani, Umaima;Leon, Rosa;Purton, Saul
萜类化合物是一类天然存在的化合物，由50,000多个结构不同的分子组成，存在于所有活着的生物体中。许多萜类化合物，特别是那些从植物中分离出来的化合物，在包括医药、农业和化妆品在内的各个商业领域都有应用。然而，这些高价值的萜类化合物在其天然宿主中的产量相对较小，其大规模生产的化学合成成本高昂且复杂。因此，利用代谢工程技术在新型生物宿主中生产这些化合物备受关注。作为一种光合作用系统，单细胞绿藻C.reinhardtii是研究最充分的模式藻类，拥有成熟的遗传操作分子工具。然而，要直接操纵莱茵哈迪尔乳杆菌中萜类化合物的生物合成途径，就必须彻底了解萜类化合物的基本代谢。为了更好地了解甲基赤藓糖醇磷酸(MEP)途径导致类萜类化合物在叶绿体中的生物合成，本研究探讨了过表达1-脱氧-D-木酮糖-5-磷酸合成酶(DXS)对可塑性下游萜类化合物的影响。我们获得了无标记的莱茵哈蒂叶绿体转化体，该转化体表达了DXS的一个额外蓝藻基因。对转基因株系中萜类化合物含量的分析表明，过量表达DXS导致叶绿素水平下降2倍，而类胡萝卜素水平表现出变化：玉米黄质和花黄素水平增加数倍，叶黄素水平下降近一半，而β-胡萝卜素和紫黄质水平没有显著变化。
Terpenoids are a diverse class of naturally occurring compounds consisting of more than 50,000 structurally different molecules and are found in all living organisms. Many terpenoid compounds, in particular those isolated from plants, have applications in various commercial sectors including medicine, agriculture and cosmetics. However, these high value terpenoids are produced in relatively small quantities in their natural hosts and their chemical synthesis for large scale production is costly and complicated. Therefore, there is much focus on producing these compounds in novel biological hosts using metabolic engineering technologies. As a photosynthetic system, the unicellular green alga C. reinhardtii is of particular interest as the most well-studied model alga with well-established molecular tools for genetic manipulation. However, the direct manipulation of terpenoid biosynthetic pathways in C. reinhardtii necessitates a thorough understanding of the basic terpenoid metabolism. To gain a better understanding of the methylerythritol phosphate (MEP) pathway that leads to terpenoid biosynthesis in the chloroplast of C. reinhardtii, hence this study has investigated the effect of over-expressing 1-deoxy-d-xylulose-5-phosphate synthase (DXS) on plastidic downstream terpenoids. We produced marker-free chloroplast transformants of C. reinhardtii lines that express an additional cyanobacterial gene for DXS. The analysis of terpenoid content for the transgenic line demonstrates that overexpressing DXS resulted in a two-fold decrease in the chlorophyll levels while carotenoid levels showed variable changes: zeaxanthin and antherxanthin levels increased several-fold, lutein levels dropped to approximately half, but β-carotene and violaxanthin did not show a significant change.
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影响因子：
4.8
作者：
Estévez, JM;Cantero, A;León, P
通讯作者：
León, P
影响因子：
4.5
作者：
通讯作者：
--
影响因子：
8.4
作者：
Lauersen, Kyle J.;Baier, Thomas;Kruse, Olaf
通讯作者：
Kruse, Olaf
DOI：
10.1007/10_2014_301
发表时间：
2015-01-01
期刊：
BIOTECHNOLOGY OF ISOPRENOIDS
影响因子：
--
作者：
Pateraki, Irini;Heskes, Allison Maree;Hamberger, Bjorn
通讯作者：
Hamberger, Bjorn
影响因子：
6.4
作者：
Alokam, S;Li, YJ;Reid, DM
通讯作者：
Reid, DM