Interactions Among Nerve Regeneration, Angiogenesis, and the Immune Response Immediately After Sciatic Nerve Crush Injury in Sprague-Dawley Rats.
Interactions Among Nerve Regeneration, Angiogenesis, and the Immune Response Immediately After Sciatic Nerve Crush Injury in Sprague-Dawley Rats.
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斯普拉格-道利大鼠坐骨神经挤压损伤后神经再生、血管生成和免疫反应之间的相互作用。
DOI：
10.3389/fncel.2021.717209
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发表时间：
2021
影响因子：
5.3
通讯作者：
Zhu Z
中科院分区：
文献类型：
--
作者：
He B;Pang V;Liu X;Xu S;Zhang Y;Djuanda D;Wu G;Xu Y;Zhu Z
为初步探讨外周神经再生、血管生成和免疫应答相关基因表达的主要变化，寻找重要的分子治疗靶点，将45只SD大鼠随机分为对照组和损伤组。损伤组在损伤后第4天和第7天采集组织样本，结合基因本体（GO）分析、京都基因与基因组百科全书（KEGG）分析和Venn图构建进行下一代测序（NGS）分析，以鉴定与神经再生、血管生成和免疫反应相关的差异表达mrna （demrna）。采用qRT-PCR分析损伤后神经远端和近端基因的表达情况。NGS显示，与对照组相比，损伤组在损伤后4天有4020个DEmRNAs，损伤后7天有3278个DEmRNAs。生物信息学分析表明,碳碳趋化因子受体5 (CCR5)类型,Thy1细胞表面抗原(Thy1),切口同族体1 (Notch1)和semaphorin 4 A (Sema4A)都与再生,血管生成,和免疫反应神经损伤后4和7天,但qPCR透露在Thy1的表达无显著差异(P = 0.29)或Sema4A近端(P = 0.82),而一个显著差异在CCR5和Notch1 (P < 0.05)。Notch1的变化趋势与损伤后RNA-seq结果基本一致，暗示其在内皮细胞增殖迁移、巨噬细胞募集、神经营养因子分泌等过程中发挥着不可或缺的作用。
To preliminarily explore the primary changes in the expression of genes involved in peripheral nerve processes, namely, regeneration, angiogenesis, and the immune response, and to identify important molecular therapeutic targets, 45 Sprague-Dawley (SD) rats were randomly divided into a control group and an injury group. In the injury group, tissue samples were collected at 4 and 7 days after the injury for next-generation sequencing (NGS) analysis combined with gene ontology (GO) analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis and Venn diagram construction to identify the differentially expressed mRNAs (DEmRNAs) associated with regeneration, angiogenesis, and the immune response of the nerve. The expression of genes in the distal and proximal ends of the injured nerve after injury was analyzed by qRT-PCR. NGS revealed that compared with the control group, the injury group had 4020 DEmRNAs 4 days after injury and 3278 DEmRNAs 7 days after injury. A bioinformatics analysis showed that C-C chemokine receptor type 5 (CCR5), Thy1 cell surface antigen (Thy1), Notch homolog 1 (Notch1), and semaphorin 4A (Sema4A) were all associated with regeneration, angiogenesis, and the immune response of the nerve at both 4 and 7 days after injury, but qPCR revealed no significant difference in the expression of Thy1 (P = 0.29) or Sema4A (P = 0.82) in the proximal end, whereas a significant difference was observed in CCR5 and Notch1 (P < 0.05). The trend in the Notch1 change was basically consistent with the RNA-seq result after injury, which implied its indispensable role during endothelial cell proliferation and migration, macrophage recruitment, and neurotrophic factor secretion.
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影响因子：
9.3
作者：
Lindborg JA;Niemi JP;Howarth MA;Liu KW;Moore CZ;Mahajan D;Zigmond RE
通讯作者：
Zigmond RE
影响因子：
6.1
作者：
He B;Zhu Z;Zhu Q;Zhou X;Zheng C;Li P;Zhu S;Liu X;Zhu J
通讯作者：
Zhu J
影响因子：
6.1
作者：
Liu JH;Tang Q;Liu XX;Qi J;Zeng RX;Zhu ZW;He B;Xu YB
通讯作者：
Xu YB
影响因子：
4.1
作者：
Sayad Fathi S;Zaminy A
通讯作者：
Zaminy A
影响因子：
14
作者：
Bozkurt, Ahmet;Deumens, Ronald;Pallua, Norbert
通讯作者：
Pallua, Norbert