Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes.
Arsenic exposure and glutamate-induced gliotransmitter release from astrocytes.
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砷暴露和谷氨酸诱导星形胶质细胞释放胶质递质
DOI：
10.3969/j.issn.1673-5374.2012.31.005
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发表时间：
2012-11-05
影响因子：
6.1
通讯作者：
Sun G
中科院分区：
文献类型：
--
作者：
Wang Y;Zhao F;Liao Y;Jin Y;Sun G
本研究使用从新生大鼠中分离的原代星形胶质细胞进行培养，以验证砷酸盐诱导的神经毒性可以通过改变谷氨酸诱导的胶质递质释放来影响神经元功能的假设。将原代星形胶质细胞分别暴露于0、2.5、5、10、20或30µM亚砷酸盐中24小时。细胞活力和形态学观察表明，5µM砷暴露可诱导细胞毒性。然后将细胞在0、2.5、5或10µM亚砷酸盐中培养24小时，并用25µM谷氨酸刺激10分钟。结果表明，5和10µM亚砷酸盐作用下星形胶质细胞[Ca2+]i显著升高，2.5 ~ 10µM亚砷酸盐作用下d -丝氨酸、γ-氨基丁酸和甘氨酸水平升高。然而，培养基中的谷氨酸水平只有在10µM亚砷酸盐处理后才显著升高。总之，我们的研究结果表明，砷暴露可能影响谷氨酸诱导的胶质递质从星形胶质细胞释放，并进一步扰乱神经元功能。
The present study used cultures of primary astrocytes, isolated from neonatal rats, to verify the hypothesis that arsenite-induced neurotoxicity can influence neuronal function by altering glutamate-induced gliotransmitter release. Primary astrocytes were exposed to 0, 2.5, 5, 10, 20 or 30 µM arsenite for 24 hours. Cell viability and morphological observations revealed that 5 µM arsenic exposure could induce cytotoxicity. Cells were then cultured in the presence of 0, 2.5, 5, or 10 µM arsenite for 24 hours and stimulated with 25 µM glutamate for 10 minutes. Results showed that [Ca2+]i in astrocytes exposed to 5 and 10 µM arsenite was significantly increased and levels of D-serine, γ-aminobutyric acid and glycine in cultures exposed to 2.5–10 µM arsenite were also increased. However, glutamate levels in the media were significantly increased only after treatment with 10 µM arsenite. In conclusion, our findings suggest that arsenic exposure may affect glutamate-induced gliotransmitter release from astrocytes and further disturb neuronal function.
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影响因子：
10.4
作者：
Wasserman GA;Liu X;Parvez F;Ahsan H;Factor-Litvak P;Kline J;van Geen A;Slavkovich V;Loiacono NJ;Levy D;Cheng Z;Graziano JH
通讯作者：
Graziano JH
DOI：
10.1523/jneurosci.5783-10.2011
发表时间：
2011-05-25
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Pirttimaki TM;Hall SD;Parri HR
通讯作者：
Parri HR
影响因子：
8.3
作者：
Calderón, J;Navarro, ME;Díaz-Barriga, F
通讯作者：
Díaz-Barriga, F
中国内蒙古地区饮用水中砷暴露的儿童和成人的尿砷代谢物。
影响因子：
10.4
作者：
Sun G;Xu Y;Li X;Jin Y;Li B;Sun X
通讯作者：
Sun X
影响因子：
4.8
作者：
Xu, Jun;Peng, Hong;Kang, Jian
通讯作者：
Kang, Jian