Spatiotemporal development of growth and death zones in expanding bacterial colonies driven by emergent nutrient dynamics
Spatiotemporal development of growth and death zones in expanding bacterial colonies driven by emergent nutrient dynamics
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由新兴营养动态驱动的不断扩大的细菌菌落中生长区和死亡区的时空发展
DOI：
10.1101/2023.08.27.554977
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
T. Hwa
中科院分区：
文献类型：
--
作者：
Harish Kannan;Paul Sun;Tolga Çaglar;Pantong Yao;Brian R. Taylor;Kinshuk Sahu;Daotong Ge;Matteo Mori;M. Warren;D. Kleinfeld;JiaJia Dong;B. Li;T. Hwa
硬琼脂上的细菌菌落生长在微生物学中是常见的;然而，即使在最简单的情况下，生长中的菌落内发生的事情也是复杂的。强大的菌落扩张动力学特征的线性径向生长和饱和的垂直生长表明一个共同的发展计划，在这里阐明了大肠杆菌细胞使用的建模和实验的组合。径向菌落扩张被发现是有限的机械因素，而不是通常假设的营养。相比之下，垂直扩展受到殖民地内葡萄糖消耗的限制，由于厌氧而导致的生长产量降低的影响。在殖民地内部的碳饥饿导致在1-2天内大量细胞死亡，具有随着生长的菌落而扩大的明显的死亡区。总的来说，缺乏EPS生产和分化的简单菌落的发展是由强制性代谢过程中产生的机械约束和紧急营养梯度的相互作用决定的。
Bacterial colony growth on hard agar is commonplace in microbiology; yet, what occurs inside a growing colony is complex even in the simplest cases. Robust colony expansion kinetics featuring a linear radial growth and a saturating vertical growth indicates a common developmental program which is elucidated here for Escherichia coli cells using a combination of modeling and experiments. Radial colony expansion is found to be limited by mechanical factors rather than nutrients as commonly assumed. In contrast, vertical expansion is limited by glucose depletion inside the colony, an effect compounded by reduced growth yield due to anaerobiosis. Carbon starvation in the colony interior results in substantial cell death within 1-2 days, with a distinct death zone that expands with the growing colony. Overall, the development of simple colonies lacking EPS production and differentiation is dictated by an interplay of mechanical constraints and emergent nutrient gradients arising from obligatory metabolic processes.
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影响因子：
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作者：
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DOI：
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发表时间：
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